Send to

Choose Destination
J Cell Mol Med. 2018 Oct 24. doi: 10.1111/jcmm.13965. [Epub ahead of print]

Intranasal immunization with Mycobacterium tuberculosis Rv3615c induces sustained adaptive CD4+ T-cell and antibody responses in the respiratory tract.

Author information

Institute of Immunology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China.
Laboratory of Infectious Diseases and Vaccine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.


Sustained adaptive immunity to pathogens provides effective protection against infections, and effector cells located at the site of infection ensure rapid response to the challenge. Both are essential for the success of vaccine development. To explore new vaccination approach against Mycobacterium tuberculosis (M.tb) infection, we have shown that Rv3615c, identified as ESX-1 substrate protein C of M.tb but not expressed in BCG, induced a dominant Th1-type response of CD4+ T cells from patients with tuberculosis pleurisy, which suggests a potential candidate for vaccine development. But subcutaneous immunization with Rv3615c induced modest T-cell responses systemically, and showed suboptimal protection against virulent M.tb challenge at the site of infection. Here, we use a mouse model to demonstrate that intranasal immunization with Rv3615c induces sustained capability of adaptive CD4+ T- and B-cell responses in lung parenchyma and airway. Rv3615c contains a dominant epitope of mouse CD4+ T cells, Rv3615c41-50 , and elicits CD4+ T-cell response with an effector-memory phenotype and multi-Th1-type cytokine coexpressions. Since T cells resident at mucosal tissue are potent at control of infection at early stage, our data show that intranasal immunization with Rv3615c promotes a sustained regional immunity to M.tb, and suggests a potency in control of M.tb infection. Our study warranties a further investigation of Rv3615c as a candidate for development of effective vaccination against M.tb infection.


Mycobacterium tuberculosis ; CD4; Rv3615c; Th1; adaptive immunity; intranasal; mucosal

Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center