Format

Send to

Choose Destination
Mol Microbiol. 2019 Jan;111(1):287-301. doi: 10.1111/mmi.14155. Epub 2018 Dec 2.

A Bifidobacterial pilus-associated protein promotes colonic epithelial proliferation.

Author information

1
APC Microbiome Ireland, National University of Ireland, Western Road, Cork, Ireland.
2
School of Microbiology, National University of Ireland, Western Road, Cork, Ireland.
3
Department of Medicine, National University of Ireland, Cork University Hospital, Cork, Ireland.
4
Research Programme Unit Immunobiology, Department of Bacteriology and Immunology, Helsinki University, Helsinki, Finland.
5
Cancer and Translational Medicine Research Unit and Biocenter Oulu, University of Oulu, Oulu, Finland.
6
Laboratory of Microbiology, Wageningen University, The Netherlands.

Abstract

Development of the human gut microbiota commences at birth, with certain bifidobacterial species representing dominant and early colonisers of the newborn gastrointestinal tract. The molecular basis of Bifidobacterium colonisation, persistence and presumed communication with the host has remained obscure. We previously identified tight adherence (Tad) pili from Bifidobacterium breve UCC2003 as an essential colonisation factor. Here, we demonstrate that bifidobacterial Tad pili also promote in vivo colonic epithelial proliferation. A significant increase in cell proliferation was detectable 5 days postadministration of B. breve UCC2003. Using advanced functional genomic approaches, bacterial strains either (a) producing the Tad2003 pili or (b) lacking the TadE or TadF pseudopilins were created. Analysis of the ability of these mutant strains to promote epithelial cell proliferation in vivo demonstrated that the pilin subunit, TadE, is the bifidobacterial molecule responsible for this proliferation response. These findings were confirmed in vitro using purified TadE protein. Our data imply that bifidobacterial Tad pili may contribute to the maturation of the naïve gut in early life through the production of a specific scaffold of extracellular protein structures, which stimulate growth of the neonatal mucosa.

PMID:
30352131
DOI:
10.1111/mmi.14155
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center