Send to

Choose Destination
Chem Biodivers. 2019 Jan;16(1):e1800303. doi: 10.1002/cbdv.201800303. Epub 2018 Dec 13.

Characterization of Schinus lentiscifolius Marchand (Anacardiaceae) Bark Extract and Its Effects on Lymphocyte Oxidative Stress and Heat Shock Response.

Author information

Research Group in Physiology, Postgraduate Program in Integral Attention to Health (PPGAIS-UNIJUÍ/UNICRUZ), Department of Life Sciences, Regional, University of Northwestern Rio Grande do Sul State (UNIJUÍ), Rua do Comércio, 3000, Bairro Universitário, Ijuí RS, 98700-000, Brazil.
Postgraduate Program in Integral Attention to Health (PPGAIS-UNIJUÍ/UNICRUZ), Regional University of Northwestern region of the state of Rio Grande do Sul (UNIJUÍ), Ijuí RS, 98700-000, Brazil.
Department of Chemical Sciences and Natural Resources, Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Temuco, 4811-230, Chile.


Schinus lentiscifolius Marchand has been used in folk medicine to treat immunoinflammatory related diseases, which are marked by OS and altered HSR. Our study aimed to evaluate OS and HSR in lymphocytes treated with S. lentiscifolius bark extracts. S. lentiscifolius barks were partitioned with solvents to obtain hexane (SL-HEX), ethyl acetate (SL-ACOET) and methanol (SL-MEOH) extracts, and the presence of bioactive compounds was evaluated by thin layer chromatography. Total phenols were measured by the Folin-Ciocalteu method and flavonoids were identified by HPLC-DAD-ESI-MS/MS. Antioxidant capacity was verified by DPPH method, cell viability by Trypan Blue method, lipid peroxidation by TBARS and HSP70 by immunoblotting. The SL-ACOET extract presented higher content of phenolic compounds and antioxidant activity in vitro. It was able to reduce lipid peroxidation levels in lymphocytes induced by H2 O2 and improved cell viability. The SL-ACOET extract inhibited HSR by a decrease in both intracellular content and release of 70 kDa heat shock proteins (HSP70) and also by decrease extra-to-intracellular HSP70 ratio in lymphocytes submitted to heat shock (2 h, 41 °C). S. lentiscifolius bark extract has antioxidant activity and inhibitory effect on HSR probably due to the presence of polyphenols as the flavonoids quercetin and kaempferol.


HSP70; Schinus lentiscifolius; antioxidant activity; biological activity; flavonoids; lipid peroxidation; oxidative stress

[Indexed for MEDLINE]

MeSH terms, Substances, Grant support

MeSH terms


Grant support

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center