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Elife. 2018 Oct 23;7. pii: e37857. doi: 10.7554/eLife.37857.

Acute perturbation of Pet1-neuron activity in neonatal mice impairs cardiorespiratory homeostatic recovery.

Author information

1
Department of Genetics, Harvard Medical School, Boston, United States.
2
Department of Molecular & Systems Biology, The Geisel School of Medicine at Dartmouth, Hanover, United States.

Abstract

Cardiorespiratory recovery from apneas requires dynamic responses of brainstem circuitry. One implicated component is the raphe system of Pet1-expressing (largely serotonergic) neurons, however their precise requirement neonatally for homeostasis is unclear, yet central toward understanding newborn cardiorespiratory control and dysfunction. Here we show that acute in vivo perturbation of Pet1-neuron activity, via triggering cell-autonomously the synthetic inhibitory receptor hM4Di, resulted in altered baseline cardiorespiratory properties and diminished apnea survival. Respiratory more than heart rate recovery was impaired, uncoupling their normal linear relationship. Disordered gasp recovery from the initial apnea distinguished mice that would go on to die during subsequent apneas. Further, the risk likelihood of apnea-related mortality associated with suppression of Pet1 neurons was higher for animals with baseline elevated ventilatory equivalents for oxygen. These findings establish that Pet1 neurons play an active role in neonatal cardiorespiratory homeostasis and provide mechanistic plausibility for the serotonergic abnormalities associated with SIDS.

KEYWORDS:

SIDS; autoresuscitation; chemogenetics; human biology; medicine; mouse; neonatal; neuroscience; raphe; serotonergic system

PMID:
30350781
PMCID:
PMC6199134
DOI:
10.7554/eLife.37857
[Indexed for MEDLINE]
Free PMC Article

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