Send to

Choose Destination
Methods Mol Biol. 2019;1872:11-22. doi: 10.1007/978-1-4939-8823-5_2.

Quantification of Eight Cannabinoids Including Cannabidiol in Human Urine Via Liquid Chromatography Tandem Mass Spectrometry.

Author information

Quest Diagnostics, Chantilly, VA, USA.
Quest Diagnostics, Chantilly, VA, USA.


Medical and recreational cannabis legalization has highlighted the importance of being able to identify recent cannabis use and impairment. Monitoring minor plant cannabinoids has been proposed to assist in identifying recent cannabis use. Additionally, cannabidiol (CBD) has been proposed for epilepsy, pain, inflammatory disorder, anxiety, and addiction treatment; therefore, monitoring CBD is of increasing clinical importance. However, few methods exist capable of monitoring extensive panels of traditional cannabinoid analytes and minor cannabinoids (including CBD). This chapter details a liquid chromatography tandem mass spectrometry method capable of measuring Δ9-tetrahydrocannabinol (THC), 11-hydroxy-THC, 11-nor-9-carboxy-THC, cannabinol, cannabigerol, tetrahydrocannabivarin (THCV), and its metabolite, 11-nor-9-carboxy-THCV, in urine.


Cannabidiol; Cannabinoids; Liquid chromatography; Mass spectrometry; Pipette tip extraction; Urine

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center