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Proc Natl Acad Sci U S A. 2018 Nov 6;115(45):E10539-E10547. doi: 10.1073/pnas.1810819115. Epub 2018 Oct 22.

Intestinal barrier dysfunction orchestrates the onset of inflammatory host-microbiome cross-talk in a human gut inflammation-on-a-chip.

Author information

1
Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712.
2
Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712; hyunjung.kim@utexas.edu.
3
Department of Medical Engineering, Yonsei University College of Medicine, 03722 Seoul, Republic of Korea.

Abstract

The initiation of intestinal inflammation involves complex intercellular cross-talk of inflammatory cells, including the epithelial and immune cells, and the gut microbiome. This multicellular complexity has hampered the identification of the trigger that orchestrates the onset of intestinal inflammation. To identify the initiator of inflammatory host-microbiome cross-talk, we leveraged a pathomimetic "gut inflammation-on-a-chip" undergoing physiological flow and motions that recapitulates the pathophysiology of dextran sodium sulfate (DSS)-induced inflammation in murine models. DSS treatment significantly impaired, without cytotoxic damage, epithelial barrier integrity, villous microarchitecture, and mucus production, which were rapidly recovered after cessation of DSS treatment. We found that the direct contact of DSS-sensitized epithelium and immune cells elevates oxidative stress, in which the luminal microbial stimulation elicited the production of inflammatory cytokines and immune cell recruitment. In contrast, an intact intestinal barrier successfully suppressed oxidative stress and inflammatory cytokine production against the physiological level of lipopolysaccharide or nonpathogenic Escherichia coli in the presence of immune elements. Probiotic treatment effectively reduced the oxidative stress, but it failed to ameliorate the epithelial barrier dysfunction and proinflammatory response when the probiotic administration happened after the DSS-induced barrier disruption. Maintenance of epithelial barrier function was necessary and sufficient to control the physiological oxidative stress and proinflammatory cascades, suggesting that "good fences make good neighbors." Thus, the modular gut inflammation-on-a-chip identifies the mechanistic contribution of barrier dysfunction mediated by intercellular host-microbiome cross-talk to the onset of intestinal inflammation.

KEYWORDS:

barrier function; disease model; gut inflammation-on-a-chip; inflammation; microbiome

PMID:
30348765
PMCID:
PMC6233106
[Available on 2019-05-06]
DOI:
10.1073/pnas.1810819115

Conflict of interest statement

The authors declare no conflict of interest.

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