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PLoS One. 2018 Oct 22;13(10):e0205306. doi: 10.1371/journal.pone.0205306. eCollection 2018.

Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix.

Author information

1
Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom.
2
Institute of Pathophysiological Anesthesiology and Process Engineering, University of Ulm, Ulm, Germany.
3
Institute of General Physiology, University of Ulm, Ulm, Germany.

Abstract

A range of cellular functions have been attributed to caveolae, flask-like invaginations of the plasma membrane. Here, we have used RNA-seq to achieve quantitative transcriptional profiling of primary embryonic fibroblasts from caveolin 1 knockout mice (CAV1-/- MEFs), and thereby to gain hypothesis-free insight into how these cells respond to the absence of caveolae. Components of the extracellular matrix were decisively over-represented within the set of genes displaying altered expression in CAV1-/- MEFs when compared to congenic wild-type controls. This was confirmed biochemically and by imaging for selected examples. Up-regulation of components of the extracellular matrix was also observed in a second cell line, NIH-3T3 cells genome edited to delete CAV1. Up-regulation of components of the extracellular matrix was detected in vivo by assessing collagen deposition and compliance of CAV1-/- lungs. We discuss the implications of these findings in terms of the cellular function of caveolae.

PMID:
30346954
DOI:
10.1371/journal.pone.0205306
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Conflict of interest statement

The authors have declared that no competing interests exist.

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