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Environ Microbiol. 2018 Nov;20(11):3952-3963. doi: 10.1111/1462-2920.14419. Epub 2018 Oct 22.

Environment-driven changes of mRNA and protein levels in Pseudomonas aeruginosa.

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Centre for Experimental and Clinical Infection Research, a joint venture of the Hannover Medical School and the Helmholtz Centre for Infection Research, Institute for Molecular Bacteriology, TWINCORE GmbH, Hannover, Germany.
Centre for Pharmacology and Toxicology, Research Core Unit Proteomics and Institute of Toxicology, Hannover Medical School, Hannover, Germany.
Department of Molecular Bacteriology, Helmholtz Center for Infection Research, Braunschweig, Germany.


Systems biology approaches address the challenge of translating sequence information into function. In this study, we described the Pseudomonas aeruginosa PA14 proteomic landscape and quantified environment-driven changes in protein levels by the use of LC-MS techniques. Previously recorded mRNA data allowed a comparison of RNA to protein ratios for each individual gene and, thus, to explore the relationship between an mRNA being differentially expressed between environmental conditions and the mRNA-protein correlation for that gene. We developed a Random Forest-based predictor for protein levels and found that the mRNA to protein correlation was higher for genes/proteins that undergo dynamic changes. One example of a discrepancy between protein and predicted protein levels was observed for a phage-related gene cluster, which was translated into low protein levels under standard growth conditions. However, under SOS-inducing conditions more protein was produced and the prediction of protein levels based on mRNA abundancy became more accurate. In conclusion, our systems biology approach sheds light on complex mRNA to protein level relationships and uncovered condition-dependent post-transcriptional regulatory events.

[Indexed for MEDLINE]

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