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Int J Stem Cells. 2018 Nov 30;11(2):187-195. doi: 10.15283/ijsc18057.

MiR-9 Controls Chemotactic Activity of Cord Blood CD34⁺ Cells by Repressing CXCR4 Expression.

Author information

1
Soonchunhyang Institute of Medi-bio Science (SIMS), Soon Chun Hyang University, Cheonan, Korea.
2
Department of Obstetrics and Gynecology, Soon Chun Hyang University College of Medicine, Bucheon, Korea.
3
Department of Obstetrics and Gynecology, Soon Chun Hyang University Cheonan Hospital, Cheonan, Korea.
4
Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, USA.

Abstract

Improved approaches for promoting umbilical cord blood (CB) hematopoietic stem cell (HSC) homing are clinically important to enhance engraftment of CB-HSCs. Clinical transplantation of CB-HSCs is used to treat a wide range of disorders. However, an improved understanding of HSC chemotaxis is needed for facilitation of the engraftment process. We found that ectopic overexpression of miR-9 and antisense-miR-9 respectively down- and up-regulated C-X-C chemokine receptor type 4 (CXCR4) expression in CB-CD34 cells as well as in 293T and TF-1 cell lines. Since CXCR4 is a specific receptor for the stromal cell derived factor-1 (SDF-1) chemotactic factor, we investigated whether sense miR-9 and antisense miR-9 influenced CXCR4-mediated chemotactic mobility of primary CB CD34 cells and TF-1 cells. Ectopic overexpression of sense miR-9 and antisense miR-9 respectively down- and up-regulated SDF-1-mediated chemotactic cell mobility. To our knowledge, this study is the first to report that miR-9 may play a role in regulating CXCR4 expression and SDF-1-mediated chemotactic activity of CB CD34 cells.

KEYWORDS:

CXCR4; Cell migration; Cord blood; microRNA

PMID:
30343551
DOI:
10.15283/ijsc18057
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