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Int J Biol Macromol. 2019 Jan;121:936-946. doi: 10.1016/j.ijbiomac.2018.10.125. Epub 2018 Oct 19.

Hemostasis and anti-necrotic activity of wound-healing dressing containing chitosan nanoparticles.

Author information

1
Department of Fibres and Textile Processing Technology, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai 400 019, India.
2
Department of Pharmaceutical Sciences & Technology, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai 400 019, India.
3
Department of Fibres and Textile Processing Technology, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai 400 019, India. Electronic address: rv.adivarekar@ictmumbai.edu.in.

Abstract

Necrotic tissues are the dead tissues present in the wounded areas, which need to be removed for rapid wound healing. Various biopolymer-based dressings have been exploited to heal infected wounds, but with limited success. In a quest to develop an effective and economic wound dressing, a biodegradable dressing containing chitosan nanoparticles has been successfully developed. Chitosan nanoparticles were prepared by ionic gelation method and then assembled into the porous chitosan dressing, by lyophilization. The resulting dressing was analyzed for morphology, porosity, pore volume, surface area and biodegradability. Higher surface area and porosity of the dressing facilitated its partial biodegradation by enzymatic action. In vitro cellular investigations with Human Dermal Fibroblasts (HDF) confirmed the safety of the dressing for wound healing applications. Human Thrombin-Antithrombin (TAT) based in vitro ELISA assay, for evaluating the hemostasis activity, illustrated an accelerated hemostasis activity, through higher thrombin generation and stable blood clot formation. The blood in contact with the dressing contained two-fold higher levels of TAT, as compared to that in contact with the TAT standard. Our results suggest the potential of the developed dressing for removing the necrotic tissues and accelerating the hemostasis activity, for efficient and rapid wound healing.

KEYWORDS:

Cell proliferation; Chitosan dressing; Hemostasis

PMID:
30342937
DOI:
10.1016/j.ijbiomac.2018.10.125
[Indexed for MEDLINE]

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