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Chin J Integr Med. 2018 Dec;24(12):912-919. doi: 10.1007/s11655-018-2566-5. Epub 2018 Oct 19.

Petiveria alliacea Suppresses Airway Inflammation and Allergen-Specific Th2 Responses in Ovalbumin-Sensitized Murine Model of Asthma.

Author information

1
Natural Products Research Laboratory, Higher School of Chemical Engineering and Extractive Industries, Instituto Politecnico Nacional (IPN) Professional Unit Adolfo Lopez Mateos Av. Instituto Politécnico Nacional S /N, cp, 07708, Mexico City, Mexico.
2
Natural Products Research Laboratory, Higher School of Chemical Engineering and Extractive Industries, Instituto Politecnico Nacional (IPN) Professional Unit Adolfo Lopez Mateos Av. Instituto Politécnico Nacional S /N, cp, 07708, Mexico City, Mexico. rmpg@prodigy.net.mx.

Abstract

OBJECTIVE:

To examine the effect of metanol extract of Petiveria alliacea (PM) on airway inflflammation in a murine model of chronic asthma.

METHODS:

Two-month-old male BALB/c mice (n=6-8/group) were sensitized on days 0 and 14 by intraperitoneal injection of 20 μg ovalbumin (OVA). On day 25, the mice received an airway challenge with OVA (3%, w/v, in phosphate buffered saline). PM was administered orally by oral gavage to mice at doses of 100, 200 and 400 mg/kg body weight once daily from days 18 to 23. Control mice were orally administered phosphate buffered saline (PBS) to induce a model of asthma. At the end of the test, respiratory reactivity was assayed, the total cell number, interleukin-4 (IL-4), IL-5, IL-13, tumor necrosis factor-alpha (TNF-α) and reactive oxygen species (ROS) in the bronchoalveolar lavage fluid (BALF) were determined and the levels of serum IgE, intercellular cell adhesion molecule 1 (ICAM-1) and eotoxin were measured. In addition, lung tissue was used to qualify the IL-4, IL-5, IL-13, TNF-α and transforming growth factor beta 1 (TGF-β1). Histologic examination was performed to observe inflammatory cellular infiltration.

RESULTS:

The administration of PM in comparison with the OVA-only treated group signifificantly attenuated the infifiltration of eosinophils and other inflflammatory cells (P<0.01). Airway resistance (RI) in the OVA-only induced group was significantly higher than that of the PBS control group (P<0.01) when methacholine was added. TNF-α, IgE, TGF-β1 and cytokine levels IL-4, IL-5, IL-13 in the BALF decreased compared to control mice (P<0.01 or P<0.05). PM treatment also inhibited the production of chemokines, eotaxin and ICAM-1 in BALF (P<0.01), which improved lung function. Histopathological examination revealed that the sensitized treated PM groups had significant lower in inflammatory scores similar to dexamethasone treatments and the untreated group.

CONCLUSION:

Administration of PM could inhibit airway inflammation, regulate cytokines, chemokines and enhance pulmonary conditions in allergic murine model of asthma.

KEYWORDS:

Petiveria alliacea; allergy; antiinflflammation; asthma

PMID:
30341485
DOI:
10.1007/s11655-018-2566-5
[Indexed for MEDLINE]

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