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Reprod Biol Endocrinol. 2018 Oct 19;16(1):100. doi: 10.1186/s12958-018-0420-4.

Frequency of the T307A, N680S, and -29G>A single-nucleotide polymorphisms in the follicle-stimulating hormone receptor in Mexican subjects of Hispanic ancestry.

Author information

1
Instituto Valenciano de Infertilidad (IVINSEMER), Thiers 96, Col. Nueva Anzurez, CP 11590, Mexico City, Mexico.
2
Red de Apoyo a la Investigación (RAI), Coordinación de la Investigación Científica, Universidad Nacional Autónoma de México (UNAM)-Instituto Nacional de Ciencias Médicas y Nutrición SZ (INCMNSZ), Calle Vasco de Quiroga 15, Tlalpan, 14000, Ciudad de Mexico, Mexico.
3
Centro de Investigación en Salud Poblacional, Instituto Nacional de Salud Pública, Av. Universidad 655, CP 62100, Cuernavaca, Mor, Mexico.
4
Department of Physiology of Nutrition, INCMNSZ, Mexico City, Mexico.
5
Research Unit in Reproductive Medicine, UMAE Hospital de Ginecoobstetricia "Luis Castelazo Ayala", Río de la Magdalena 289, Tizapán San Ángel, Mexico City, 01090, Mexico.
6
Facultad de Medicina, UNAM, Ciudad Universitaria, Mexico City, Mexico.
7
Department of Transplantation, INCMNSZ, Mexico City, Mexico.
8
Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, Leyes de Reforma 1ra. Secc., Mexico City, 09340, Mexico.
9
Unidad de Biología Molecular y Medicina Genómica, UNAM-INCMNSZ, Mexico City, Mexico.
10
Red de Apoyo a la Investigación (RAI), Coordinación de la Investigación Científica, Universidad Nacional Autónoma de México (UNAM)-Instituto Nacional de Ciencias Médicas y Nutrición SZ (INCMNSZ), Calle Vasco de Quiroga 15, Tlalpan, 14000, Ciudad de Mexico, Mexico. aulloaa@unam.mx.

Abstract

BACKGROUND:

FSHR SNPs may influence the ovarian sensitivity to endogenous and exogenous FSH stimulation. Given the paucity of data on the FSHR c.919A > G, c.2039A > G and - 29G > A SNPs in Hispanic population, we here analyzed their frequency distribution in Mexican mestizo women.

METHODS:

Samples from 224 Mexican mestizo women enrolled in an IVF program as well as a genotype database from 8182 Mexican mestizo subjects, were analyzed for FSHR SNPs at positions c.919, c.2039 and - 29G > A. Association between the genetic variants and reproductive outcomes was assessed.

RESULTS:

The c.919 and c.2039 SNPs were in strong linkage disequilibrium and their corresponding genotype frequencies in the IVF group were: AA 46.8%, AG 44.2%, and GG 8.9%, and AA 41.9%, AG 48.2% and GG 9.8%, respectively. For the -29G > A SNP, genotype frequencies were 27% (GG), 50% (GA) and 23% (AA). In normal oocyte donors with the c.2039 GG genotype, the number of oocytes recovered after ovarian stimulation (COS) were significantly (p < 0.01) lower than in those bearing other genotypes in this or the -29G > A SNP. Analysis of the large scale database revealed that both allelic and genotype frequencies for the three SNPs were very similar to those detected in the IVF cohort (p ≥ 0.38) and that female carriers of the c.2039 G allele tended to present lower number of pregnancies than women bearing the AA genotype; this trend was stronger when women with more Native American ancestry was separately analyzed (OR = 2.0, C.I. 95% 1.03-3.90, p = 0.04). There were no differences or trends in the number of pregnancies among the different genotypes of the -29G > A SNP.

CONCLUSIONS:

The frequency of the GG/GG combination genotype for the c.919 and c.2039 SNPs in Mexican hispanics is among the lowest reported. The GG genotype is associated with decreased number of oocytes recovered in response to COS as well as to lower pregnancy rates in Hispanic women from the general population. The absence of any effect of the -29AA genotype on the response to COS, indicates that there is no need to perform this particular genotype testing in Hispanic women with the purpose of providing an individually-tailored COS protocol.

KEYWORDS:

Assisted reproductive techniques; FSHR gene SNPs; Female infertility; Follicle stimulating hormone; Infertility; Oocyte donation; Ovulation induction

PMID:
30340493
PMCID:
PMC6195735
DOI:
10.1186/s12958-018-0420-4
[Indexed for MEDLINE]
Free PMC Article

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