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Photochem Photobiol. 2018 Oct 19. doi: 10.1111/php.13037. [Epub ahead of print]

Spectral Properties of Fluorogenic Thiophene-Derived Triarylmethane Dyes.

Author information

1
Departments of Chemistry and Biological Sciences.
2
Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania, 15213.
3
Molecular Biosensor and Imaging Center.

Abstract

Spectral properties and fluorogenic behaviors of five novel thiophene variants of Malachite Green (MG), termed MGTs, were determined. Appreciable changes as a function of homologation and substitution pattern, including absorption band positions and intensities and fluorescence quantum yields were observed. In particular, the shorter wavelength y-band absorption was found to shift over a nearly 200 nm range based on aryl group variation, allowing fine-tuning of the excitation wavelength for these dyes. In addition, the fluorescence intensity of some MGTs increased significantly (up to 4,600-fold) when the dye was bound to a cognate protein partner, which is potentially useful for cell imaging studies. This article is protected by copyright. All rights reserved.

PMID:
30339278
DOI:
10.1111/php.13037

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