Format

Send to

Choose Destination
FEMS Microbiol Ecol. 2019 Jan 1;95(1). doi: 10.1093/femsec/fiy210.

Multilocus sequence typing of Shewanella algae isolates identifies disease-causing Shewanella chilikensis strain 6I4.

Author information

1
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Solnavägen 9, 171 65 Solna, Sweden.
2
Departamento de Bioquímica, Microbiología, Biología Molecular y Genética, Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Avenida Astrofísico Francisco Sánchez s/n, 38206 La Laguna, Spain.
3
Hospital Universitario de Gran Canaria "Doctor Negrín", Barranco de la Ballena s/n, 35010 Las Palmas de Gran Canaria, Spain.

Abstract

The genus Shewanella is rapidly expanding, with new species being discovered frequently. Four species have been identified as pathogenic to humans, with Shewanella algae being most relevant. Evaluation of the clinical significance of Shewanella spp. still suffers from the imprecision of species identification. In addition, the origin of S. algae strains causing disease is unclear. To shed light upon these questions we re-identified reported S. algae isolates on the species level based on the analysis of the partial sequences of the 16S rRNA and gyrB genes in combination with multilocus sequence typing that included six housekeeping loci. The analysis of a collection of 23 S. algae isolates of clinical and environmental origin, the publicly available genome sequences of six additional S. algae strains and type strains of closely-related species showed the existence of a remarkable haplotypic diversity within the S. algae clade. Three of the analyzed strains are suggested to be assigned to a species different from S. algae. A clinical isolate was thus reclassified as S. chilikensis, thereby constituting the first known case of human infection by this species. Our study emphasizes the application of high resolution molecular markers for species identification. The taxonomic resolution of the S. algae clade is still unclear.

PMID:
30339183
DOI:
10.1093/femsec/fiy210

Supplemental Content

Full text links

Icon for Silverchair Information Systems
Loading ...
Support Center