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Plant Cell. 2018 Oct 18. pii: tpc.00292.2018. doi: 10.1105/tpc.18.00292. [Epub ahead of print]

Patterned deposition of xylan and lignin is independent from the secondary wall cellulose of Arabidopsis xylem vessels.

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Ritsumeikan University CITY: Kusatsu Japan [JP].
University of British Columbia CITY: Vancouver Canada [CA].
University of British Columbia CITY: Vancouver POSTAL_CODE: V6T1Z4 Canada [CA].
Department of Wood Science, University of British Columbia CITY: Vancouver Canada [CA].
Penn State CITY: University Park STATE: PA POSTAL_CODE: 16802 United States Of America [US].
Nara Institute of Science and Technology CITY: Ikoma Japan [JP].
University of British Columbia CITY: Vancouver STATE: BC POSTAL_CODE: V6T 1Z4 Canada [CA].
NAIST CITY: Ikoma STATE: Nara POSTAL_CODE: 630-0192 Japan [JP].
Nara Institute of Science and Technology CITY: Ikoma POSTAL_CODE: 630-0192 Japan [JP]


The secondary cell wall (SCW) of xylem vessel cells provides rigidity and strength that enables efficient water conduction throughout the plant. To gain insight into SCW deposition, we mutagenized Arabidopsis thaliana VASCULAR-RELATED NAC-DOMAIN7-inducible plant lines, in which ectopic protoxylem vessel cell differentiation is synchronously induced. The baculites mutant was isolated based on the absence of helical SCW patterns in ectopically-induced protoxylem vessel cells, and mature baculites plants exhibited an irregular xylem (irx) mutant phenotype in mature plants. A single nucleic acid substitution in the CELLULOSE SYNTHASE SUBUNIT 7 (CESA7) gene in baculites was identified: while the mutation was predicted to produce a C-terminal truncated protein, immunoblot analysis revealed that cesa7bac mutation results in loss of production of CESA7 proteins, indicating that baculites is a novel cesa7 loss-of-function mutant. In cesa7bac, despite a lack of patterned cellulose deposition, the helically-patterned deposition of other SCW components, such as the hemicellulose xylan and the phenolic polymer lignin, was not affected. Similar phenotypes were found in another point mutation mutant cesa7mur10-2, and an established knock-out mutant, cesa7irx3-4. Taken together, we propose that the spatio-temporal deposition of different SCW components, such as xylan and lignin, is not dependent on cellulose patterning.

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