Responsiveness extracellular electron transfer (EET) enhancement of anaerobic digestion system during start-up and starvation recovery stages via magnetite addition

Caiqin Wang; Yang Liu; Shuai Jin; Hui Chen; Xiangyang Xu; Zhirong Wang; Bo Xing; Liang Zhu

doi:10.1016/j.biortech.2018.10.013

Responsiveness extracellular electron transfer (EET) enhancement of anaerobic digestion system during start-up and starvation recovery stages via magnetite addition

Bioresour Technol. 2019 Jan:272:162-170. doi: 10.1016/j.biortech.2018.10.013. Epub 2018 Oct 5.

Authors

Caiqin Wang¹, Yang Liu¹, Shuai Jin¹, Hui Chen¹, Xiangyang Xu², Zhirong Wang³, Bo Xing⁴, Liang Zhu⁵

Affiliations

¹ Institute of Environment Pollution Control and Treatment, Zhejiang University, Hangzhou 310058, China.
² Institute of Environment Pollution Control and Treatment, Zhejiang University, Hangzhou 310058, China; Zhejiang Province Key Laboratory for Water Pollution Control and Environmental Safety, Hangzhou 310058, China; Zhejiang Provincial Engineering Laboratory of Water Pollution Control, 388 Yuhangtang Road, Hangzhou 310058, China.
³ Zhejiang Provincal Office of Agricultural Ecology and Energy, Hangzhou 310012, China.
⁴ Shaoxing Environmental Monitoring Center, Shaoxing 312000, China.
⁵ Institute of Environment Pollution Control and Treatment, Zhejiang University, Hangzhou 310058, China; Zhejiang Province Key Laboratory for Water Pollution Control and Environmental Safety, Hangzhou 310058, China; Zhejiang Provincial Engineering Laboratory of Water Pollution Control, 388 Yuhangtang Road, Hangzhou 310058, China. Electronic address: felix79cn@zju.edu.cn.

PMID: 30336398
DOI: 10.1016/j.biortech.2018.10.013

Abstract

Two up-flow anaerobic sludge blanket (UASB) reactors (RM with 10 g Fe L^-1 magnetite, RB without magnetite) feeding with synthetic wastewater were built to investigate the effect of magnetite addition on anaerobic digestion (AD) performance during start-up and starvation recovery stages. With the magnetite addition, the COD removal efficiency and biogas production during the two stages were enhanced, and the recovery time of RM was shortened by about 50%. The reduced synthesis of riboflavin and heme c along with enhanced sludge conductivity of RM indicated that magnetite could replace their roles for efficient extracellular electron transfer (EET), which favors the growth of anaerobes. Microbial community analysis showed that potential syntrophic partners like Syntrophaceae and Methanothrix were enriched in RM during the recovery stage, and the performance was improved with quick responsiveness. Results demonstrated that addition of conductive materials like magnetite could improve the stability and restorability of AD process efficiently.

Keywords: Anaerobic digestion; Extracellular electron transfer (EET); Magnetite; Methanothrix; Starvation.

MeSH terms

Anaerobiosis
Bacteria, Anaerobic / metabolism
Electron Transport*
Electrons
Extracellular Space
Ferrosoferric Oxide*
Sewage

Substances

Sewage
Ferrosoferric Oxide