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ACS Sens. 2018 Nov 26;3(11):2296-2302. doi: 10.1021/acssensors.8b00558. Epub 2018 Nov 5.

Non-invasive Point-of-Care Device To Diagnose Acute Mesenteric Ischemia.

Author information

1
Department of Electrical and Computer Engineering , University of Alberta , Edmonton , Alberta T6G 1H9 , Canada.
2
Department of Surgery, Faculty of Medicine & Dentistry , University of Alberta , Edmonton , Alberta T6G 2B7 , Canada.
3
Department of Medicine, Faculty of Medicine & Dentistry , University of Alberta , Edmonton , Alberta T6G 2G3 , Canada.
4
Department of Internal Medicine , Yale University School of Medicine , New Haven , Connecticut 06510 , United States.
5
Department of Biological Sciences , University of Alberta , Edmonton , Alberta T6G 2E9 , Canada.
6
Department of Computing Science , University of Alberta , Edmonton , Alberta T6G 2E8 , Canada.

Abstract

Inadequate blood supply to the intestine can lead to acute mesenteric ischemia (AMI), with a mortality rate ranging from 60% to 90%. This high mortality rate is partially due to late detection and the lack of efficient early diagnostic tests. There is an urgent need for a point-of-care tool for immediate bedside diagnosis. Here we present for the first time a rapid and non-invasive electrochemical biosensor device based on non-faradic impedance spectroscopy to detect intestinal fatty-acid binding protein (I-FABP) as an indication of AMI. The electrochemical biosensors consist of gold interdigitated electrodes that were fabricated using photolithographic techniques on top of silicon dioxide substrates. The electrode surfaces were functionalized with an I-FABP capture antibody (CAnB) to entice the target protein, while gold nanoparticles (GNPs) functionalized with detection antibodies (DAnB-GNPs) were utilized as a novel mechanism to enhance the detection signal. Quantification of the I-FABP concentration in the medium depended on its attachment to CAnB and DAnB-GNPs in a sandwich manner, where the latter boosts the impedance signal through its binding to the I-FABP. This non-invasive non-faradic electric biosensor device demonstrates the potential for bench-to-bedside translation with the goal of decreasing morbidity and mortality from AMI.

KEYWORDS:

acute mesenteric ischemia; bedside monitoring; impedance-based biosensor; non-invasive urine test; point-of-care device

PMID:
30335977
DOI:
10.1021/acssensors.8b00558

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