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Genome Biol Evol. 2018 Nov 1;10(11):2997-3011. doi: 10.1093/gbe/evy232.

Genome Sequence of the Wheat Stem Sawfly, Cephus cinctus, Representing an Early-Branching Lineage of the Hymenoptera, Illuminates Evolution of Hymenopteran Chemoreceptors.

Author information

1
Department of Entomology, University of Illinois at Urbana-Champaign.
2
Department of Ecology and Evolution, University of Lausanne, Switzerland.
3
Swiss Institute of Bioinformatics, Lausanne, Switzerland.
4
United States Department of Agriculture, Agricultural Research Service (USDA ARS), Corn Insects & Crop Genetics Research Unit, Ames, Iowa.
5
Ecology and Genetics of Insects, INRA, UMR IGEPP 1349, Rennes, France.
6
Department of Science and Mathematics, Abraham Baldwin Agricultural College, Tifton, Georgia.
7
Department of Plant Sciences and Plant Pathology, Montana State University.
8
Department of Land Resources and Environmental Sciences, Montana State University.

Abstract

The wheat stem sawfly, Cephus cinctus, is a major pest of wheat and key ecological player in the grasslands of western North America. It also represents the distinctive Cephoidea superfamily of sawflies (Symphyta) that appeared early during the hymenopteran radiation, but after three early-branching eusymphytan superfamilies that form the base of the order Hymenoptera. We present a high-quality draft genome assembly of 162 Mb in 1,976 scaffolds with a scaffold N50 of 622 kb. Automated gene annotation identified 11,210 protein-coding gene models and 1,307 noncoding RNA models. Thirteen percent of the assembly consists of ∼58,000 transposable elements partitioned equally between Class-I and Class-II elements. Orthology analysis reveals that 86% of Cephus proteins have identifiable orthologs in other insects. Phylogenomic analysis of conserved subsets of these proteins supports the placement of the Cephoidea between the Eusymphyta and the parasitic woodwasp superfamily Orussoidea. Manual annotation and phylogenetic analysis of families of odorant, gustatory, and ionotropic receptors, plus odorant-binding proteins, shows that Cephus has representatives for most conserved and expanded gene lineages in the Apocrita (wasps, ants, and bees). Cephus has also maintained several insect gene lineages that have been lost from the Apocrita, most prominently the carbon dioxide receptor subfamily. Furthermore, Cephus encodes a few small lineage-specific chemoreceptor gene family expansions that might be involved in adaptations to new grasses including wheat. These comparative analyses identify gene family members likely to have been present in the hymenopteran ancestor and provide a new perspective on the evolution of the chemosensory gene repertoire.

PMID:
30335145
PMCID:
PMC6250288
DOI:
10.1093/gbe/evy232
[Indexed for MEDLINE]
Free PMC Article

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