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Methods Mol Biol. 2019;1834:345-364. doi: 10.1007/978-1-4939-8669-9_22.

Analysis of the Drosophila Compound Eye with Light and Electron Microscopy.

Author information

1
National Institute of Technology Rourkela (NITR), Rourkela, Odisha, India.
2
Max-Planck-Institute of Molecular Cell Biology and Genetics, Dresden, Germany. knust@mpi-cbg.de.

Abstract

The Drosophila compound eye is composed of about 750 units, called ommatidia, which are arranged in a highly regular pattern. Eye development proceeds in a stereotypical fashion, where epithelial cells of the eye imaginal discs are specified, recruited, and differentiated in a sequential order that leads to the highly precise structure of an adult eye. Even small perturbations, for example in signaling pathways that control proliferation, cell death, or differentiation, can impair the regular structure of the eye, which can be easily detected and analyzed. In addition, the Drosophila eye has proven to be an ideal model for studying the genetic control of neurodegeneration, since the eye is not essential for viability. Several human neurodegeneration diseases have been modeled in the fly, leading to a better understanding of the function/misfunction of the respective gene. In many cases, the genes involved and their functions are conserved between flies and human. More strikingly, when ectopically expressed in the fly eye some human genes, even those without a Drosophila counterpart, can induce neurodegeneration, detectable by aberrant phototaxis, impaired electrophysiology, or defects in eye morphology and retinal histology. These defects are often rather subtle alteration in shape, size, or arrangement of the cells, and can be easily scored at the ultrastructural level. This chapter aims to provide an overview regarding the analysis of the retina by light and electron microscopy.

KEYWORDS:

Compound eye; Cryolabeling; Deep pseudopupil; Drosophila melanogaster; Electron microscopy; Light microscopy; Whole mount

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