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Elife. 2018 Oct 15;7. pii: e37551. doi: 10.7554/eLife.37551.

Species and cell-type properties of classically defined human and rodent neurons and glia.

Author information

1
Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, United States.
2
Miller School of Medicine, University of Miami, Miami, United States.

Abstract

Determination of the molecular properties of genetically targeted cell types has led to fundamental insights into mouse brain function and dysfunction. Here, we report an efficient strategy for precise exploration of gene expression and epigenetic events in specific cell types in a range of species, including postmortem human brain. We demonstrate that classically defined, homologous neuronal and glial cell types differ between rodent and human by the expression of hundreds of orthologous, cell specific genes. Confirmation that these genes are differentially active was obtained using epigenetic mapping and immunofluorescence localization. Studies of sixteen human postmortem brains revealed gender specific transcriptional differences, cell-specific molecular responses to aging, and the induction of a shared, robust response to an unknown external event evident in three donor samples. Our data establish a comprehensive approach for analysis of molecular events associated with specific circuits and cell types in a wide variety of human conditions.

KEYWORDS:

RNA-seq; cerebellum; chromosomes; gene expression; human; human molecular variation; molecular evolution; mouse; neuroscience; rat; tools and resources

PMID:
30320555
PMCID:
PMC6188473
DOI:
10.7554/eLife.37551
[Indexed for MEDLINE]
Free PMC Article

Conflict of interest statement

XX XX is named as an inventor on a patent application filed on this technology, assigned to The Rockefeller University (application number PCT/US2018/017556). XX is currently an employee at a startup company using this technology. Conduct of the studies presented in this manuscript were not influenced by these relationships. ES, AL, JX, DM No competing interests declared, NH NH is named as an inventor on a patent application filed on this technology, assigned to The Rockefeller University (application number PCT/US2018/017556). NH is a founder of a startup company using this technology. Conduct of the studies presented in this manuscript were not influenced by these relationships.

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