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Med Gas Res. 2018 Sep 25;8(3):79-84. doi: 10.4103/2045-9912.241064. eCollection 2018 Jul-Sep.

Hydrogen-rich water ameliorates rat placental stress induced by water restriction.

Author information

1
Department of Basic Stomatology and Technology, School of Stomatology, Taishan Medical University, Taishan, Shandong Province, China.
2
Department of Stomatology, Taian City Central Hospital, Taishan, Shandong Province, China.
3
Key Laboratory of Atherosclerosis in Universities of Shandong and Institute of Atherosclerosis, Taishan Medical University, Taishan, Shandong Province, China.

Abstract

Dehydration is one of the intrauterine abnormalities that could lead to fetal growth retardation and to increase the risk of a variety of adult diseases later in life. This study were to determine the impact of hydrogen-rich water (HRW) supplementation on placental angiotensin II type 1 receptor and placental oxidative stress induced by water restriction. Pregnant Wistar rat were randomly assigned to one of the three groups (n =12 per group). In control group, pure water and food were supplied ad libitum. Water restriction group and HRW group were respectively given pure water and HRW with free access to food, excepting only one hour was available for drinking from day 7 to day 17 of pregnancy. The placental damages and biomarkers of stress were detected by histopathology, immunohistochemistry and western blot, as well as serological test were performed. We demonstrated that maternal water restriction resulted in reduced urine volume and increased serum osmotic pressure, along with decreased fetus weight and crown-rump length. Although placental weight and the number of fetuses had no significant difference among groups, the placental efficiency significantly increased after the oral administration of HRW to the mothers. Meanwhile, the serological derivatives of reactive oxygen metabolites decreased, a significant improvement of placental microstructure with more developed junctional zone and denser labyrinth was manifested, the upregulated expression of angiotensin II type 1 receptor, nuclear factoκB, malondialdehyde, 8-hydroxydeoxyguanosine, p38, c-Jun N-terminal kinase and down-regulation of superoxide dismutase were revealed in the placenta. Collectively, HRW administration is able to effectively attenuate placental stress induced by water restriction.

KEYWORDS:

AT1R; dehydration; fetus; growth retardation; hydrogen-rich water; oxidative stress; placenta; rat

Conflict of interest statement

Conflicts of interest The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported.

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