Send to

Choose Destination
Front Physiol. 2018 Sep 26;9:1205. doi: 10.3389/fphys.2018.01205. eCollection 2018.

Ethanol Extract of Aurantiochytrium mangrovei 18W-13a Strain Possesses Anti-inflammatory Effects on Murine Macrophage RAW264 Cells.

Author information

Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan.
Alliance for Research on the Mediterranean and North Africa, University of Tsukuba, Tsukuba, Japan.
Algae Biomass and Energy System R&D Center, University of Tsukuba, Tsukuba, Japan.


In this study, the effects of an ethanolic extract of Aurantiochytrium mangrovei 18W-13a strain (AM18W-13a) on lipopolysaccharide (LPS)-induced inflammatory responses in RAW264 murine macrophages were studied. Pre-treatment with the AM18W-13a extract significantly suppressed the LPS-induced production of nitric oxide and pro-inflammatory cytokines. RAW264 cells treated with the AM18W-13a extract for 1 and 24 h were subjected to DNA microarray analyses for detecting the differentially expressed genes. The treatment of RAW264 cells with the AM18W-13a extract for 24 h significantly suppressed the expression of several genes associated with inflammation or chemotaxis. Furthermore, treatment with the AM18W-13a extract for 1 h suppressed the expression of Pde4b, but induced the expression of Egr2 and Egr3 in RAW264 cells. Additionally, the AM18W-13a extract significantly enhanced the expression of certain anti-inflammatory mediators. This study is the first report of the anti-inflammatory effects of the AM18W-13a extract and its mechanism of action in LPS-stimulated murine macrophages.


Aurantiochytrium; RAW264 cells; anti-inflammation; lipopolysaccharide; microalgae; nitric oxide; pro-inflammatory cytokines

Supplemental Content

Full text links

Icon for Frontiers Media SA Icon for PubMed Central
Loading ...
Support Center