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Front Mol Neurosci. 2018 Sep 27;11:325. doi: 10.3389/fnmol.2018.00325. eCollection 2018.

BDNF-Live-Exon-Visualization (BLEV) Allows Differential Detection of BDNF Transcripts in vitro and in vivo.

Author information

1
Department of Otolaryngology, Tübingen Hearing Research Centre (THRC), Molecular Physiology of Hearing, University of Tübingen, Tübingen, Germany.
2
Department of Pharmacology, Institute of Pharmacy, Toxicology and Clinical Pharmacy, University of Tübingen, Tübingen, Germany.
3
B.R.A.I.N. Centre for Neuroscience, Department of Life Sciences, University of Trieste, Trieste, Italy.
4
Centre for Neuroscience and Cell Biology (CNC), Department of Life Sciences, University of Coimbra, Coimbra, Portugal.
5
Centre for Integrative Neuroscience (CIN), University of Tübingen, Tübingen, Germany.
6
Department of Physiology, Institute of Physiology, University of Hohenheim, Stuttgart, Germany.
7
Transgenic Facility Tübingen, University of Tübingen, Tübingen, Germany.
8
Instituto de Biologíay Genética Molecular, Universidad de Valladolid y Consejo Superior de Investigaciones Científicas, Valladolid, Spain.

Abstract

Bdnf exon-IV and exon-VI transcripts are driven by neuronal activity and are involved in pathologies related to sleep, fear or memory disorders. However, how their differential transcription translates activity changes into long-lasting network changes is elusive. Aiming to trace specifically the network controlled by exon-IV and -VI derived BDNF during activity-dependent plasticity changes, we generated a transgenic reporter mouse for B DNF- l ive- e xon- v isualization (BLEV), in which expression of Bdnf exon-IV and -VI can be visualized by co-expression of CFP and YFP. CFP and YFP expression was differentially activated and targeted in cell lines, primary cultures and BLEV reporter mice without interfering with BDNF protein synthesis. CFP and YFP expression, moreover, overlapped with BDNF protein expression in defined hippocampal neuronal, glial and vascular locations in vivo. So far, activity-dependent BDNF cannot be explicitly monitored independent of basal BDNF levels. The BLEV reporter mouse therefore provides a new model, which can be used to test whether stimulus-induced activity-dependent changes in BDNF expression are instrumental for long-lasting plasticity modifications.

KEYWORDS:

BDNF detection; Bdnf exon-IV and -VI; activity-dependent BDNF expression; long-lasting plasticity changes; transgenic BDNF reporter mice

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