Format

Send to

Choose Destination
Microbes Environ. 2018 Dec 28;33(4):428-434. doi: 10.1264/jsme2.ME18058. Epub 2018 Oct 12.

Nitric Oxide Production from Nitrite Reduction and Hydroxylamine Oxidation by Copper-containing Dissimilatory Nitrite Reductase (NirK) from the Aerobic Ammonia-oxidizing Archaeon, Nitrososphaera viennensis.

Author information

1
Department of Civil Engineering, National Institute of Technology, Nagaoka College.
2
Department of Applied Life Science, Faculty of Biotechnology and Life Science, Sojo University.
3
Graduate School of Life and Environmental Sciences, University of Tsukuba.
4
Department of Bioengineering, Nagaoka University of Technology.
5
Department of Science of Technology Innovation, Nagaoka University of Technology.

Abstract

Aerobic ammonia-oxidizing archaea (AOA) play a crucial role in the global nitrogen cycle by oxidizing ammonia to nitrite, and nitric oxide (NO) is a key intermediate in AOA for sustaining aerobic ammonia oxidation activity. We herein heterologously expressed the NO-forming, copper-containing, dissimilatory nitrite reductase (NirK) from Nitrososphaera viennensis and investigated its enzymatic properties. The recombinant protein catalyzed the reduction of 15NO2- to 15NO, the oxidation of hydroxylamine (15NH2OH) to 15NO, and the production of 14-15N2O from 15NH2OH and 14NO2-. To the best of our knowledge, the present study is the first to document the enzymatic properties of AOA NirK.

KEYWORDS:

Nitrososphaera viennensis; ammonia oxidizing archaea; hydroxylamine oxidation; nitrite reduction; nitrous oxide production

PMID:
30318500
PMCID:
PMC6308003
DOI:
10.1264/jsme2.ME18058
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for J-STAGE, Japan Science and Technology Information Aggregator, Electronic Icon for PubMed Central
Loading ...
Support Center