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Cell. 2018 Oct 18;175(3):780-795.e15. doi: 10.1016/j.cell.2018.09.012. Epub 2018 Oct 11.

Centromeres License the Mitotic Condensation of Yeast Chromosome Arms.

Author information

1
Institute of Biochemistry, Biology Department, ETH Zurich, 8093 Zurich, Switzerland.
2
Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Montebello, 0379 Oslo, Norway.
3
Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Montebello, 0379 Oslo, Norway; Faculty of Medicine, Center for Cancer Cell Reprogramming, Institute of Clinical Medicine, University of Oslo, Oslo, Norway; Faculty of Mathematics and Natural Sciences, Department of Biosciences, University of Oslo, Norway.
4
Institute of Biochemistry, Biology Department, ETH Zurich, 8093 Zurich, Switzerland. Electronic address: yves.barral@bc.biol.ethz.ch.

Abstract

During mitosis, chromatin condensation shapes chromosomes as separate, rigid, and compact sister chromatids to facilitate their segregation. Here, we show that, unlike wild-type yeast chromosomes, non-chromosomal DNA circles and chromosomes lacking a centromere fail to condense during mitosis. The centromere promotes chromosome condensation strictly in cis through recruiting the kinases Aurora B and Bub1, which trigger the autonomous condensation of the entire chromosome. Shugoshin and the deacetylase Hst2 facilitated spreading the condensation signal to the chromosome arms. Targeting Aurora B to DNA circles or centromere-ablated chromosomes or releasing Shugoshin from PP2A-dependent inhibition bypassed the centromere requirement for condensation and enhanced the mitotic stability of DNA circles. Our data indicate that yeast cells license the chromosome-autonomous condensation of their chromatin in a centromere-dependent manner, excluding from this process non-centromeric DNA and thereby inhibiting their propagation.

KEYWORDS:

Aurora B; DNA circle; Shugoshin; centromere; chromatin; chromosome; chromosome condensation; foreign DNA; nucleosome deacetylation; nucleosomes

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