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Ultrasound Med Biol. 2019 Jan;45(1):129-136. doi: 10.1016/j.ultrasmedbio.2018.08.023. Epub 2018 Oct 8.

Testing Different Combinations of Acoustic Pressure and Doses of Quinolinic Acid for Induction of Focal Neuron Loss in Mice Using Transcranial Low-Intensity Focused Ultrasound.

Author information

1
Department of Ultrasound, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science and Technology, China; Neuroradiology Section, Department of Radiology, Stanford University, Stanford, California, USA.
2
Neuroradiology Section, Department of Radiology, Stanford University, Stanford, California, USA; Department of Radiology, Third Affiliated Hospital of Kunming Medical University, Tumor Hospital of Yunnan Province, Kunming, Yunnan, China.
3
Neuroradiology Section, Department of Radiology, Stanford University, Stanford, California, USA; Department of Medical Imaging, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China.
4
Neuroradiology Section, Department of Radiology, Stanford University, Stanford, California, USA; Traditional Chinese Medicine College, Chongqing Medical University, Chongqing, China.
5
Neuroradiology Section, Department of Radiology, Stanford University, Stanford, California, USA; Department of Neurology, Peking University of People's Hospital, Beijing, China.
6
Neuroradiology Section, Department of Radiology, Stanford University, Stanford, California, USA; The Acupuncture and Tuina School/Third Teaching Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China.
7
Department of Comparative Medicine, Stanford University, Stanford, California, USA.
8
Department of Radiology, Molecular Imaging Program, Stanford University, Stanford, California, USA.
9
Department of Ultrasound, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science and Technology, China.
10
Department of Neurology, University of Virginia, Charlottesville, Virginia, USA.
11
Departments of Neuroscience and Neurosurgery and Center for Brain Immunology and Glia, University of Virginia, Charlottesville, Virginia, USA.
12
Department of Radiology, Stanford University, Stanford, California, USA.
13
Neuroradiology Section, Department of Radiology, Stanford University, Stanford, California, USA. Electronic address: max.wintermark@gmail.com.

Abstract

The goal of this study was to test different combinations of acoustic pressure and doses of quinolinic acid (QA) for producing a focal neuronal lesion in the murine hippocampus without causing unwanted damage to adjacent brain structures. Sixty male CD-1 mice were divided into 12 groups that underwent magnetic resonance-guided focused ultrasound at high (0.67 MPa), medium (0.5 MPa) and low (0.33 MPa) acoustic peak negative pressures and received QA at high (0.012 mmol), medium (0.006 mmol) and low (0.003 mmol) dosages. Neuronal loss occurred only when magnetic resonance-guided focused ultrasound with adequate acoustic power (0.67 or 0.5 MPa) was combined with QA. The animals subjected to the highest acoustic power had larger lesions than those treated with medium acoustic power, but two mice had evidence of bleeding. When the intermediate acoustic power was used, medium and high dosages of QA produced lesions larger than those produced by the low dosage.

KEYWORDS:

Blood–brain barrier; Epilepsy; Hippocampus; Magnetic resonance-guided focused ultrasound; Quinolinic acid; Transcranial drug delivery

PMID:
30309748
PMCID:
PMC6289648
[Available on 2020-01-01]
DOI:
10.1016/j.ultrasmedbio.2018.08.023
[Indexed for MEDLINE]

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