Format

Send to

Choose Destination
FEBS Lett. 1987 Mar 9;213(1):149-54.

Glucose regulates preproinsulin messenger RNA levels in a clonal cell line of simian virus 40-transformed B cells.

Abstract

In HIT-T15 cells grown in the absence of glucose, Northern blot analysis of total RNA revealed a major 0.5 kb preproinsulin (ppI) mRNA transcript which co-migrated with the mature transcript from a human insulinoma. In 4 h tissue cultures, glucose (2-20 mM) stimulated HIT cell ppI mRNA levels in a markedly dose-dependent manner. Glucose-stimulated ppI mRNA was (i) inhibited by actinomycin D, suggesting that regulation may be in part transcriptional, and (ii) potentiated by agents known to activate B cell protein kinases. HIT cells represent a unique model for investigating long term regulation of insulin gene expression and biosynthesis.

PMID:
3030804
DOI:
10.1016/0014-5793(87)81481-3
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center