Format

Send to

Choose Destination
J Phycol. 2019 Feb;55(1):118-133. doi: 10.1111/jpy.12798. Epub 2018 Nov 26.

Acutely induced cell mortality in the unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae) following exposure to acrylic resin nanoparticles.

Author information

1
School of Environmental Science and Engineering, Kochi University of Technology, 185 Miyanokuchi, Tosayamada, Kami City, Kochi, 782-8502, Japan.
2
Chikami Miltec Inc, 1-6-3 Ohtesuji, Kochi City, Kochi, 780-0842, Japan.
3
Graduate School of Science and Technology, Niigata University, 8050 Ikarashi 2-no-cho, Nishi-ku, Niigata, 950-2181, Japan.
4
Chemistry Department, Institut Teknologi Bandung, Jalan Ganesha 10, Bandung, West Java, 40132, Indonesia.

Abstract

Nanoparticles have unique properties that make them attractive for use in industrial and medical technology industries but can also be harmful to living organisms, making an understanding of their molecular mechanisms of action essential. We examined the effect of three different sized poly(isobutyl-cyanoacrylate) nanoparticles (iBCA-NPs) on the unicellular green alga Chlamydomonas reinhardtii. We found that exposure to iBCA-NPs immediately caused C. reinhardtii to display abnormal swimming behaviors. Furthermore, after one hour, most of the cells had stopped swimming and 10%-30% of cells were stained with trypan blue, suggesting that these cells had severely impaired plasma membranes. Observation of the cyto-ultrastructure showed that the cell walls had been severely damaged and that many iBCA-NPs were located in the space between the cell wall and plasma membrane, as well as inside the cytosol in some cases. A comparison of three strains of C. reinhardtii with different cell wall conditions further showed that the cell mortality ratio increased more rapidly in the absence of a cell wall. Interestingly, cell mortality over time was essentially identical regardless of iBCA-NP size if the total surface area was the same. Furthermore, direct observation of the trails of iBCA-NPs indicated that the first trigger was their contact with the cell wall, which is most likely accompanied by the inactivation or removal of adsorbed proteins from the cell wall surface. Cell mortality was accompanied by the overproduction of reactive oxygen species, which was detected more readily in cells grown under constant light rather than in the dark.

KEYWORDS:

Chlamydomonas reinhardtii ; ROS ; abnormal swimming behavior; cell mortality; cell wall damage; poly(isobutyl-cyanoacrylate) nanoparticles

PMID:
30304548
DOI:
10.1111/jpy.12798

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center