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FASEB J. 2019 Feb;33(2):2680-2693. doi: 10.1096/fj.201800431R. Epub 2018 Oct 10.

HuR stabilizes a polyadenylated form of replication-dependent histone mRNAs under stress conditions.

Ryu I1,2, Park Y1,2, Seo JW3, Park OH1,2, Ha H1,2, Nam JW3,4, Kim YK1,2.

Author information

1
Creative Research Initiatives Center for Molecular Biology of Translation, Korea University, Seoul, South Korea.
2
Division of Life Sciences, Korea University, Seoul, South Korea.
3
Department of Life Science, College of Natural Sciences, Hanyang University, Seoul, South Korea; and.
4
Research Institute for Natural Sciences, Hanyang University, Seoul, South Korea.

Abstract

All metazoan mRNAs have a poly(A) tail at the 3' end with the exception of replication-dependent histone (RDH) mRNAs, which end in a highly conserved stem-loop (SL) structure. However, a subset of RDH mRNAs are reported to be polyadenylated under physiologic conditions. The molecular details of the biogenesis of polyadenylated RDH [poly(A)+ RDH] mRNAs remain unknown. In this study, our genome-wide analyses reveal that puromycin treatment or UVC irradiation stabilizes poly(A)+ RDH mRNAs, relative to canonical RDH mRNAs, which end in an SL structure. We demonstrate that the stabilization of poly(A)+ RDH mRNAs occurs in a translation-independent manner and is regulated via human antigen R (HuR) binding to the extended 3' UTR under stress conditions. Our data suggest that HuR regulates the expression of poly(A)+ RDH mRNAs.-Ryu, I., Park, Y., Seo, J.-W., Park, O. H., Ha, H., Nam, J.-W., Kim, Y. K. HuR stabilizes a polyadenylated form of replication-dependent histone mRNAs under stress conditions.

KEYWORDS:

3′UTR; UVC; mRNA stability; polyadenylation signal

PMID:
30303743
DOI:
10.1096/fj.201800431R
[Indexed for MEDLINE]

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