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Indian J Pathol Microbiol. 2018 Oct-Dec;61(4):510-515. doi: 10.4103/IJPM.IJPM_788_16.

Diagnostic utility of CD10 immunohistochemical staining on cellblock in differentiating hepatocellular carcinoma from secondary malignancies of liver.

Author information

1
Department of Pathology, North Bengal Medical College, Siliguri, West Bengal, India.
2
Department of Urology, CNMCH, Kolkata, West Bengal, India.

Abstract

Background:

Differentiation of hepatocellular carcinoma (HCC) from metastatic malignancy in liver may be difficult at times on fine-needle aspiration cytology, especially in case of moderate-to-poorly differentiated tumors. The benefit of cell-block technique is the recognition of histologic pattern of diseases along with application of a wide variety of immunohistochemical (IHC) stains to differentiate hepatic malignancies. In this study, CD10 IHC staining was done on cellblocks prepared from aspirates of clinicoradiologically/cytologically suspected malignant liver neoplasms to differentiate HCC from malignancies metastasizing to liver.

Objective:

The objective of the study was to assess the diagnostic utility of CD10 IHC stain on cell-block preparation for differentiating primary from Secondary malignancies of liver.

Materials and Methods:

Formalin-fixed, paraffin-embedded cellblocks of 61 cases (25 cases of HCC and 36 cases of metastatic carcinoma) were prepared from a fine-needle aspirate of the suspected malignant liver neoplasm and immunostained using monoclonal antibody against CD10.

Results:

Twenty-two (88%) of 25 cases of HCC were positive for CD10 with a canalicular staining pattern. Two (8%) were positive for CD10 with membranous and one (4%) with cytoplasmic staining pattern.

Conclusion:

CD10 immunostaining on cellblock is useful in discriminating HCC and metastatic carcinoma of the liver with a diagnostic accuracy of 88.52%.

KEYWORDS:

CD10; Canalicular; cellblock; fine-needle aspiration cytology; hepatocellular carcinoma; metastatic malignancy of liver

PMID:
30303139
DOI:
10.4103/IJPM.IJPM_788_16
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