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Lab Chip. 2018 Dec 7;18(23):3550-3560. doi: 10.1039/c8lc00605a. Epub 2018 Oct 10.

An integrated adipose-tissue-on-chip nanoplasmonic biosensing platform for investigating obesity-associated inflammation.

Author information

1
Department of Mechanical and Aerospace Engineering, New York University, New York, NY, USA. wchen@nyu.edu.
2
Materials Research and Education Center, Materials Engineering, Department of Mechanical Engineering, Auburn University, Auburn, AL, USA.
3
Laboratory of Translational Obesity Research, Division of Endocrinology, Department of Medicine, New York University School of Medicine, New York, NY, USA.
4
Department of Mechanical and Aerospace Engineering, New York University, New York, NY, USA. wchen@nyu.edu and Division of Engineering, New York University Abu Dhabi, Abu Dhabi, United Arab Emirates.
5
Department of Mechanical and Aerospace Engineering, New York University, New York, NY, USA. wchen@nyu.edu and Department of Biomedical Engineering, New York University, New York, NY, USA.

Abstract

Although many advanced biosensing techniques have been proposed for cytokine profiling, there are no clinically available methods that integrate high-resolution immune cell monitoring and in situ multiplexed cytokine detection together in a biomimetic tissue microenvironment. The primary challenge arises due to the lack of suitable label-free sensing techniques and difficulty for sensor integration. In this work, we demonstrated a novel integration of a localized-surface plasmon resonance (LSPR)-based biosensor with a biomimetic microfluidic 'adipose-tissue-on-chip' platform for an in situ label-free, high-throughput and multiplexed cytokine secretion analysis of obese adipose tissue. Using our established adipose-tissue-on-chip platform, we were able to monitor the adipose tissue initiation, differentiation, and maturation and simulate the hallmark formation of crown-like structures (CLSs) during pro-inflammatory stimulation. With integrated antibody-conjugated LSPR barcode sensor arrays, our platform enables simultaneous multiplexed measurements of pro-inflammatory (IL-6 and TNF-α) and anti-inflammatory (IL-10 and IL-4) cytokines secreted by the adipocytes and macrophages. As a result, our adipose-tissue-on-chip platform is capable of identifying stage-specific cytokine secretion profiles from a complex milieu during obesity progression, highlighting its potential as a high-throughput preclinical readout for personalized obesity treatment strategies.

PMID:
30302487
PMCID:
PMC6246809
[Available on 2019-12-07]
DOI:
10.1039/c8lc00605a
[Indexed for MEDLINE]

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