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Dent Mater. 2018 Nov;34(11):e289-e300. doi: 10.1016/j.dental.2018.08.304. Epub 2018 Oct 6.

Towards quantitative analysis of enamel erosion by focused ion beam tomography.

Author information

1
Univ. Paris Diderot, AP-HP, Hôpital Rothschild, UFR Odontologie, Paris, France; Univ. Lyon, Université Claude Bernard Lyon 1, Laboratoire des Multimatériaux et Interfaces, UMR CNRS 5615, 69008 Lyon, France. Electronic address: dr.cougot.nancie@gmail.com.
2
Univ. Lyon, INSA-LYON, MATEIS, UMR CNRS 5510, F69621 Villeurbanne, France.
3
Univ. Paris Diderot, AP-HP, Hôpital Rothschild, UFR Odontologie, Paris, France; Univ. Lyon, Université Claude Bernard Lyon 1, Laboratoire des Multimatériaux et Interfaces, UMR CNRS 5615, 69008 Lyon, France.
4
Univ. Lyon, Université Claude Bernard Lyon 1, IFSTTAR, LBMC UMR_T9406, F69622 Lyon, France.
5
Univ. Lyon, Université Claude Bernard Lyon 1, UFR d'Odontologie, Service de Traitements et de Consultations Dentaires, Hospices Civils de Lyon, Laboratoire des Multimatériaux et Interfaces, UMR CNRS 5615, 69008 Lyon, France.

Abstract

OBJECTIVES:

The purpose of this work is a proof of concept to introduce a new quantitative 3D-analysis of dental erosion obtained by focused ion beam (FIB) tomography associated with silver nitrate penetration into porosities in etched enamel.

METHODS:

One sample selected was sound enamel after removal of the aprismatic surface. The other was studied after applying an additional attack with orthophosphoric acid. Both surfaces were infiltrated with silver nitrate via immersion. After dehydration, samples were observed in a dual column FIB/SEM station. Serial FIB sectioning was conducted with a current of 3nA at 30keV and an increment step of 20nm for the healthy enamel and of 40nm for the etched one. 3D analysis was performed with Fiji software and BoneJ plugin and several parameters were obtained to characterize the tissue: non-mineralized phase content (NMP), connected porosity fraction (CPF) and degree of anisotropy (DA) of the NMP.

RESULTS:

Healthy enamel showed an NMP content of 0.5vol.%, with a bimodal distribution of non-mineralized regions, inside the prisms and between the prisms. No silver penetration was noticed in the healthy enamel, demonstrating the absence of open porosity. In contrast, silver nitrate penetration after acidic exposure was observed, up to a depth of 12μm, which allowed the calculation of an interconnected porosity volume fraction (CPF) of 3.1vol.%, mostly between the prisms. Values for DA of 0.56 for sound enamel and 0.81 for acid-etched surface were determined, highlighting a higher degree of anisotropy in the latter.

SIGNIFICANCE:

Quantitative analysis of FIB tomography using NMP, CPF and DA should contribute to a better understanding and follow up of dental erosion, correlation between erosion and attrition or abrasion process, and the ability to develop enamel remineralization procedures.

KEYWORDS:

Demineralization; Erosion; Scanning electron microscopy; Tooth wear; Ultrastructure

PMID:
30301623
DOI:
10.1016/j.dental.2018.08.304
[Indexed for MEDLINE]

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