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Anim Reprod Sci. 2018 Nov;198:184-192. doi: 10.1016/j.anireprosci.2018.09.018. Epub 2018 Sep 29.

Effect of length of time post-mortem on quality and freezing capacity of Cantabric chamois (Rupicapra pyrenaica parva) epididymal spermatozoa.

Author information

1
Department of Clinical and Experimental Medicine (IKE), BKH, Obstetrics and Gynecology, Linköping University, Linköping, Sweden; Department of Animal Health and Anatomy, Veterinary Faculty, Universitat Autònoma de Barcelona, Barcelona, Spain.
2
Department of Medicine, Surgery and Veterinary Anatomy, Universidad de León, León, Spain; INDEGSAL, Universidad de León, 24071 León, Spain.
3
Department of Medicine, Surgery and Veterinary Anatomy, Universidad de León, León, Spain.
4
INDEGSAL, Universidad de León, 24071 León, Spain; Department of Molecular Biology (Cell Biology), Universidad de León, Spain.
5
INDEGSAL, Universidad de León, 24071 León, Spain; Department of Molecular Biology (Cell Biology), Universidad de León, Spain. Electronic address: felipe.martinez@unileon.es.

Abstract

Genome Resource Banks are keystones in the ex-situ conservation of wild species. Post-mortem (PM) collection of epididymal spermatozoa is an opportunistic and valuable source of germplasm, the time from the death of the animal limits its use. Seeking to improve germplasm preservation strategies for the chamois (Rupicapra sp.), the effect of PM time on epididymal sperm quality and freezability was studied using the Cantabrian chamois. Samples were classified according to PM collection time, up to 216 h (refrigerated), and cryopreserved (Tris-citric acid-fructose, 430 mOsm/kg, 15% egg yolk, 8% glycerol; freezing at -20 °C/min). Sperm quality was assessed after recovery and post-thawing (motility by CASA, HOS test, abnormal forms, cytoplasmic droplets, and viability and acrosomal damage by flow cytometry). The sperm mass pH and osmolality showed a positive correlation with time. Total sperm motility dropped after 2 days PM, with progressivity and sperm velocities remained similar up to 3 days PM. Sperm freezability was acceptable, with the post-thawing HOST, motility, progressivity, VAP, VCL, VSL and BCF negatively correlating with PM time. Overall, chamois epidydimal samples were not adequate for preservation after 6 days PM. Freezability capacity could make these spermatozoa suitable for specific ART even if kept refrigerated for several days PM.

KEYWORDS:

Cantabric chamois; Cryopreservation; Epididymal sperm; Germplasm banking; Post-mortem time

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