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Mol Immunol. 2018 Nov;103:191-199. doi: 10.1016/j.molimm.2018.09.020. Epub 2018 Oct 6.

Using single cell analysis for translational studies in immune mediated diseases: Opportunities and challenges.

Author information

1
Department of Immunology and Microbiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA.
2
Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, USA.
3
Department of Immunology and Microbiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA. Electronic address: lteyton@scripps.edu.

Abstract

The difficulty of studying small tissue samples and rare cell populations have been some of the main limitations in performing efficient translational studies of immune mediated diseases. Many of these conditions are grouped under the name of a single disease whilst there are strong suggestions that disease heterogeneity leads to variable disease progression as well as therapeutic responses. The recent development of single cell techniques, such as single cell RNA sequencing, gene expression profiling, or multiparametric cytometry, is likely to be a turning point. Single cell approaches provide researchers the opportunity to finally dissect disease pathology at a level that will allow mechanistic classifications and precision therapeutic strategies. In this review, we will give an overview of the current and developing repertoire of single cell techniques, the benefits and limitations of each, and provide an example of how single cell techniques can be utilized to understand complex immune mediated diseases and their translation from mouse to human.

KEYWORDS:

Immune mediated diseases; MAIT cells; Single cell analysis; Translational studies; Ulcerative colitis

PMID:
30300798
PMCID:
PMC6258053
DOI:
10.1016/j.molimm.2018.09.020
[Indexed for MEDLINE]
Free PMC Article

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