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Ultrastruct Pathol. 2018 Sep-Oct;42(5):409-415. doi: 10.1080/01913123.2018.1522406. Epub 2018 Oct 9.

Atorvastatin induces autophagy in MDA-MB-231 breast cancer cells.

Author information

1
a Department of Breast and Thyroid Surgery, Zhongnan Hospital, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center , Wuhan University , Wuhan , Hubei , China.
2
b Department of Pathology and Pathophysiology, Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences , Wuhan University , Wuhan , Hubei , China.

Abstract

This article explores the effects of atorvastatin on cultured breast cancer cells. Our experiment demonstrated that atorvastatin triggered autophagy and inhibited proliferation in breast cancer cells. A CCK8 assay indicated that atorvastatin can inhibit the activity of MDA-MB-231 breast cancer cells. Western blotting results showed that atorvastatin increased the conversion of light chain 3 (LC3)-I to LC3-phosphatidylethanolamine conjugate (LC3-II). Confocal microscopy was used to reveal the appearance of a punctate structure in the cytoplasm, and electron microscopy was used to reveal the formation of double-membrane autophagosome. In conclusion, our study showed that atorvastatin may affect MDA-MB-231 breast cancer cells by inducing autophagy.

KEYWORDS:

Atorvastatin; MDA-MB-231; autophagy; ultrastructural changes

PMID:
30300062
DOI:
10.1080/01913123.2018.1522406
[Indexed for MEDLINE]

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