Format

Send to

Choose Destination
Nucleic Acids Res. 1987 Jan 26;15(2):771-84.

DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy.

Abstract

We have determined the sequence of the E. coli gyrB gene, using a new sequencing approach in which transposition from a mini-Mu plasmid into the DNA provides random start points for dideoxynucleotide sequence analysis. The gyrB sequence corresponds to a protein 804 amino acids long; a previously isolated protein fragment with partial enzymatic activity has been identified as the C-terminal half-molecule. A plausible terminator of gyrB transcription is located just beyond the structural gene.

PMID:
3029692
PMCID:
PMC340466
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center