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Nucleic Acids Res. 1987 Jan 26;15(2):465-75.

Escherichia coli rep gene: sequence of the gene, the encoded helicase, and its homology with uvrD.

Abstract

The sequence of a 2.67-kilobase section of the Escherichia coli chromosome that contains the rep gene has been determined. This gene codes for a protein of predicted Mr 72,800, a DNA helicase, which is also a single-stranded DNA-dependent ATPase. The sequenced region contains an open reading frame of the correct length and orientation to encode the Rep protein. A secondary structure for the protein can be formulated from the amino acid sequence. We have compared both the primary and the secondary structures of Rep with other proteins and find the greatest homology between Rep and E. coli helicase II, the product of the uvrD gene.

PMID:
3029683
PMCID:
PMC340446
DOI:
10.1093/nar/15.2.465
[Indexed for MEDLINE]
Free PMC Article

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