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PLoS One. 2018 Oct 8;13(10):e0204152. doi: 10.1371/journal.pone.0204152. eCollection 2018.

Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide.

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Key Laboratory of Polysaccharide Drug Engineering of Anhui, Wannan Medical College, Wuhu, Anhui, P. R. China.
College of Life Sciences, Anhui Normal University, Wuhu, P. R. China.
College of Information Engineering, Changsha Medical University, Changsha, Hunan, P. R. China.
College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, P. R. China.
Department of Mathematics, Shanghai Normal University, Shanghai, P. R. China.
Icahn Institute for Genomics and Multiscale Biology, Icahn School of Medicine at Mount Sinai, New York, NY, United States of America.


Lonicera japonica is a typical Chinese herbal medicine. We previously reported a method to isolate polysaccharides from Lonicera japonica (LJP). In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method. We then investigated the immunomodulatory function of LJP in cyclophosphamide (CTX)-induced immunosuppressed mouse models. The results showed that LJP had the characteristic absorption of typical polysaccharides consisting of 6 types of monosaccharides. In addition, LJP can increase significantly the organ index, splenic lymphocyte proliferation, macrophage phagocytosis, and natural killer (NK) cell activity in CTX-treated mice. LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice. Finally, the results on measuring the T-lymphocytes subsets of spleen also confirmed LJP-induced immunomodulatory activity in immunosuppressed mice from another perspective. Therefore, LJP could be used as a potential immunomodulatory agent.

Conflict of interest statement

The authors have declared that no competing interests exist.

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