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PLoS One. 2018 Oct 8;13(10):e0204152. doi: 10.1371/journal.pone.0204152. eCollection 2018.

Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide.

Author information

1
Key Laboratory of Polysaccharide Drug Engineering of Anhui, Wannan Medical College, Wuhu, Anhui, P. R. China.
2
College of Life Sciences, Anhui Normal University, Wuhu, P. R. China.
3
College of Information Engineering, Changsha Medical University, Changsha, Hunan, P. R. China.
4
College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, P. R. China.
5
Department of Mathematics, Shanghai Normal University, Shanghai, P. R. China.
6
Icahn Institute for Genomics and Multiscale Biology, Icahn School of Medicine at Mount Sinai, New York, NY, United States of America.

Abstract

Lonicera japonica is a typical Chinese herbal medicine. We previously reported a method to isolate polysaccharides from Lonicera japonica (LJP). In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method. We then investigated the immunomodulatory function of LJP in cyclophosphamide (CTX)-induced immunosuppressed mouse models. The results showed that LJP had the characteristic absorption of typical polysaccharides consisting of 6 types of monosaccharides. In addition, LJP can increase significantly the organ index, splenic lymphocyte proliferation, macrophage phagocytosis, and natural killer (NK) cell activity in CTX-treated mice. LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice. Finally, the results on measuring the T-lymphocytes subsets of spleen also confirmed LJP-induced immunomodulatory activity in immunosuppressed mice from another perspective. Therefore, LJP could be used as a potential immunomodulatory agent.

Conflict of interest statement

The authors have declared that no competing interests exist.

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