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Anal Chim Acta. 2018 Dec 11;1037:168-176. doi: 10.1016/j.aca.2018.01.044. Epub 2018 Feb 9.

Software-aided quality control of parallel reaction monitoring based quantitation of lipid mediators.

Author information

1
Division of Bioanalytical Chemistry, Research Center Borstel, Parkallee 10, Borstel, Germany.
2
Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Melchor Fernandez Almagro 3, 28029 Madrid, Spain.
3
Institute of Organic Chemistry, University of Cologne, Greinstraße 4, 50939 Köln, Germany.
4
Department of Cellular Microbiology, Research Center Borstel, Parkallee 10, Borstel, Germany.
5
Division of Bioanalytical Chemistry, Research Center Borstel, Parkallee 10, Borstel, Germany. Electronic address: dschwudke@fz-borstel.de.

Abstract

We characterized the performance of a micro-flow LC-ESI-MS2 approach to analyze lipid mediators (LMs) and polyunsaturated fatty acids (PUFA) that was optimized for SPE free lipid extraction. Tandem mass spectrometry was exclusively performed in parallel reaction monitoring (PRM) mode using TOF and Orbitrap analyzers. This acquisition strategy allowed in addition to quantitation by specific quantifier ions to perform spectrum comparisons using full MS2 spectra information of the analyte. Consequently, we developed a dedicated software SpeCS that allows to 1) process raw peak lists, 2) generate customized spectral libraries, 3) test specificity of quantifier ions and 4) perform spectrum comparisons. The dedicated scoring algorithm is based on signal matching and Spearman's rank correlation of intensities of matched signal. The algorithm was evaluated in respect of its specificity to distinguish structural related LMs on both instrument platforms. We show how high resolution mass spectrometry is beneficial to distinguish co-eluted LM isomers and provide a generalized quality control procedure for PRM. The applicability of the approach was evaluated analyzing the lipid mediator response during M. tuberculosis infection in the mouse lung.

KEYWORDS:

Lipid mediators; Liquid Chromatography–Tandem mass spectrometry; Parallel reaction monitoring; Spectral library; Spectrum comparison

PMID:
30292291
DOI:
10.1016/j.aca.2018.01.044
[Indexed for MEDLINE]

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