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Curr Microbiol. 2018 Dec;75(12):1634-1641. doi: 10.1007/s00284-018-1571-4. Epub 2018 Oct 5.

Transcriptional Regulation Between the Two Global Regulators RovA and CRP in Yersinia pestis biovar Microtus.

Author information

1
Department of Transfusion, Wuhan General Hospital of PLA, No. 627, Wuluolu, Wuchang, Wuhan, 430070, Hubei, China.
2
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China.
3
Department of Transfusion, Wuhan General Hospital of PLA, No. 627, Wuluolu, Wuchang, Wuhan, 430070, Hubei, China. z_shangen@hotmail.com.
4
School of Medicine, Jiangsu University, No. 301, Xuefulu, Jingkou, Zhenjiang, 212013, Jiangsu, China. zhangyiquanq@163.com.

Abstract

Yersinia pestis is a dangerous bacterial pathogen that can cause plague. Both RovA and cyclic AMP receptor protein (cAMP-CRP) are required for regulating biofilm- and virulence-related genes in Y. pestis. In this study, the transcriptional regulation between RovA and cAMP-CRP were analyzed by using primer extension, quantitative RT-PCR, LacZ fusion, and electrophoretic mobility shift assay. The results indicated that RovA repressed crp transcription in an indirect manner, while that RovA had no regulatory action on cyaA at the transcriptional level. In addition, cAMP-CRP did not regulate the transcription of rovA. Taken together with our previous results, complex regulatory interactions of RovA, cAMP-CRP, and PhoP/PhoQ in Y. pestis were revealed, which would promote us gain deeper understanding about coordinative modulation of biofilm- and virulence-related regulator genes.

PMID:
30291406
DOI:
10.1007/s00284-018-1571-4
[Indexed for MEDLINE]

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