Format

Send to

Choose Destination
RNA Biol. 2018;15(10):1336-1347. doi: 10.1080/15476286.2018.1532253. Epub 2018 Oct 18.

RNase Y-mediated regulation of the streptococcal pyrogenic exotoxin B.

Broglia L1,2,3,4, Materne S1,2, Lécrivain AL1,2,5, Hahnke K1,2, Le Rhun A1,2,4,5, Charpentier E1,2,3,4,5.

Author information

1
a Max Planck Unit for the Science of Pathogens , Berlin , Germany.
2
b Department of Regulation in Infection Biology , Max Planck Institute for Infection Biology , Berlin , Germany.
3
c Institute for Biology , Humboldt University , Berlin , Germany.
4
d Department of Regulation in Infection Biology , Helmholtz Centre for Infection Research , Braunschweig , Germany.
5
e The Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå Centre for Microbial Research (UCMR), Department of Molecular Biology , Umeå University , Umeå , Sweden.

Abstract

Endoribonuclease Y (RNase Y) is a crucial regulator of virulence in Gram-positive bacteria. In the human pathogen Streptococcus pyogenes, RNase Y is required for the expression of the major secreted virulence factor streptococcal pyrogenic exotoxin B (SpeB), but the mechanism involved in this regulation remains elusive. Here, we demonstrate that the 5' untranslated region of speB mRNA is processed by several RNases including RNase Y. In particular, we identify two RNase Y cleavage sites located downstream of a guanosine (G) residue. To assess whether this nucleotide is required for RNase Y activity in vivo, we mutated it and demonstrate that the presence of this G residue is essential for the processing of the speB mRNA 5' UTR by RNase Y. Although RNase Y directly targets and processes speB, we show that RNase Y-mediated regulation of speB expression occurs primarily at the transcriptional level and independently of the processing in the speB mRNA 5' UTR. To conclude, we demonstrate for the first time that RNase Y processing of an mRNA target requires the presence of a G. We also provide new insights on the speB 5' UTR and on the role of RNase Y in speB regulation.

KEYWORDS:

5′ untranslated region; RNase Y; Streptococcus pyogenes; speB; transcriptional regulation; virulence

Supplemental Content

Full text links

Icon for Taylor & Francis
Loading ...
Support Center