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Regen Eng Transl Med. 2018 Jun;4(2):92-103. doi: 10.1007/s40883-018-0052-4. Epub 2018 Apr 23.

Localized SDF-1α Delivery Increases Pro-Healing Bone Marrow-Derived Cells in the Supraspinatus Muscle Following Severe Rotator Cuff Injury.

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Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA.
School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, GA.
Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA.
George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA.
Winship Cancer Institute, Emory University, Decatur, GA.
Wilmington Health Orthopedic Medical Center, Wilmington, NC.
Department of Orthopedics, Emory University, Decatur, GA.
Atlanta Veteran's Affairs Medical Center, Decatur, GA.


To examine how the chemotactic agent stromal cell-derived factor-1alpha (SDF-1α) modulates the unique cellular milieu within rotator cuff muscle following tendon injury, we developed an injectable, heparin-based microparticle platform to locally present SDF-1α within the supraspinatus muscle following severe rotator cuff injury. SDF-1α loaded, degradable, N-desulfated heparin-based microparticles were fabricated, injected into a rat model of severe rotator cuff injury, and were retained for up to 7 days at the site. The resultant inflammatory cell and mesenchymal stem cell populations were analyzed compared to uninjured contralateral controls and, after 7 days, the fold-change in anti-inflammatory, M2-like macrophages (CD11b+CD68+CD163+, 4.3X fold-change) and mesenchymal stem cells (CD29+CD44+CD90+, 3.0X, respectively) was significantly greater in muscles treated with SDF-1α loaded microparticles than unloaded microparticles or injury alone. Our results indicate that SDF-1α loaded microparticles may be a novel approach to shift the cellular composition within the supraspinatus muscle and create a more pro-regenerative milieu, which may provide a platform to improve muscle repair following rotator cuff injury in the future.


heparin; macrophages; mesenchymal stem cells; microparticles; stromal cell-derived factor-1α

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