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J Pharm Biomed Anal. 2019 Jan 30;163:45-57. doi: 10.1016/j.jpba.2018.09.046. Epub 2018 Sep 28.

New insights into phenol and polyphenol composition of Stevia rebaudiana leaves.

Author information

1
Department of Environmental Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", Via Vivaldi 43, I-81100 Caserta, Italy. Electronic address: severina.pacifico@unina2.it.
2
Department of Environmental Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", Via Vivaldi 43, I-81100 Caserta, Italy.
3
Department of Chemical Sciences, University of Naples Federico II, Via Cinthia, 26, I-80126 Naples, Italy.
4
Department of Industrial and Information Engineering, University of Campania "Luigi Vanvitelli", Via Roma 29, I-81031 Aversa, Italy.
5
Ecoricerche srl, Via Principi Normanni 36, 81043 Capua Caserta, Italy.

Abstract

The diversity in phenols and polyphenols of stevia leaf has been simplified applying sequential fractionation techniques on its ethanol extract through ultrasound assisted maceration. Two of the fractions obtained by reverse-phase column chromatography resulted differently active in an extensive antioxidant and cytotoxic screening. Both fractions were chemically profiled by ultra-performance liquid chromatography (UHPLC) coupled with electrospray ionization (ESI) quadrupole/time-of-flight (QqTOF) mass spectrometry (MS). One of the fractions was composed mainly of chlorogenic acids and flavonol triglycosides, whereas the other was rich in flavonoids mono- and diglycosides and in their hydroxycinnamoyl derivatives. Among the fifty compounds identified, non-phenol metabolites, such as benzyl primeveroside and roseoside, as well as a lignan polyphenol (5'), are reported for the first time as constituents of the Stevia leaf.

KEYWORDS:

Bioactive constituents; Chlorogenic acids; Flavonoid glycosides; Stevia rebaudiana chemical composition; UHPLC-ESI-QqTOF-MS/MS analysis; Ultrasound assisted maceration

PMID:
30286435
DOI:
10.1016/j.jpba.2018.09.046
[Indexed for MEDLINE]

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