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Mol Cancer. 2018 Oct 3;17(1):143. doi: 10.1186/s12943-018-0880-3.

Exosome-transmitted long non-coding RNA PTENP1 suppresses bladder cancer progression.

Zheng R1,2, Du M1,2,3, Wang X1,2, Xu W4, Liang J1,2, Wang W5, Lv Q6, Qin C6, Chu H1,2, Wang M1,2, Yuan L7,8, Qian J9, Zhang Z10,11.

Author information

1
Department of Environmental Genomics, School of Public Health, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Collaborative Innovation Center for Cancer Personalized Medicine, Nanjing Medical University, 101 Longmian Avenue, Jiangning District, Nanjing, 211166, China.
2
Department of Genetic Toxicology, The Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, China.
3
Department of Biostatistics, School of Public Health, Nanjing Medical University, Nanjing, China.
4
Department of Urology, Yizheng Hospital, Drum Tower Hospital Group of Nanjing, Yizheng, People's Republic of China.
5
Department of Urology, Beijing Friendship Hospital affiliated to Capital Medical University, Beijing, People's Republic of China.
6
Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
7
Department of Urology, Jiangsu Province Hospital of TCM, 155 Hanzhong Road, Nanjing, 210029, People's Republic of China. yuanlin47@126.com.
8
Department of Integrated Traditional Chinese and Western Medicine Tumor Research Lab, Nanjing, People's Republic of China. yuanlin47@126.com.
9
Department of General Surgery, Yizheng Hospital, Drum Tower Hospital Group of Nanjing, 1 Huannan Road, Yizheng, 211900, People's Republic of China. qianjing998@163.com.
10
Department of Environmental Genomics, School of Public Health, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Collaborative Innovation Center for Cancer Personalized Medicine, Nanjing Medical University, 101 Longmian Avenue, Jiangning District, Nanjing, 211166, China. drzdzhang@gmail.com.
11
Department of Genetic Toxicology, The Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, China. drzdzhang@gmail.com.

Abstract

BACKGROUND:

Extracellular communication within the tumor microenvironment plays a critical role in tumor progression. Although exosomes can package into long non-coding RNAs (lncRNAs) to mediate extracellular communication, the role of exosomal lncRNA PTENP1 in bladder cancer (BC) remains unclear.

METHOD:

We detected PTENP1 expression between patients with BC and healthy controls; the expression occurred in tissues and exosomes from plasma. We assessed the diagnostic accuracy by the receiver operating characteristic curve (ROC) and the area under curve (AUC). Cell phenotypes and animal experiments were performed to determine the effect of exosomal PTENP1.

RESULTS:

PTENP1 was significantly reduced in BC tissues and in exosomes from plasma of patients with BC (P < 0.05). We found that PTENP1 was mainly wrapped by exosomes. Exosomal PTENP1 could distinguish patients with BC from healthy controls (AUC = 0.743; 95% confidence interval (CI) = 0.645-0.840). Normal cells secreted exosomal PTENP1 and transmitted it to BC cells, thus inhibiting the biological malignant behavior of BC cells by increasing cell apoptosis and reducing the ability to invade and migrate (P < 0.05). Exosomal PTENP1 could suppress tumor growth in vivo. Furthermore, exosomal PTENP1 mediated the expression of PTEN by competitively binding to microRNA-17.

CONCLUSION:

Exosomal PTENP1 is a promising novel biomarker that can be used for the clinical detection of BC. Exosomes derived from normal cells transfer PTENP1 to BC cells, which reduce the progression of BC both in vitro and in vivo and suggest that exosomal PTENP1 participates in normal-cell-to-bladder-cell communication during the carcinogenesis of BC.

KEYWORDS:

Biomarker; Bladder cancer; Exosomes; PTENP1; Progression

PMID:
30285771
PMCID:
PMC6169076
DOI:
10.1186/s12943-018-0880-3
[Indexed for MEDLINE]
Free PMC Article

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