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BMC Cancer. 2018 Oct 1;18(1):940. doi: 10.1186/s12885-018-4840-5.

Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.

Author information

1
Cancer Science Institute of Singapore, National University of Singapore, Singapore, Singapore.
2
Cancer Science Institute of Singapore, National University of Singapore, Singapore, Singapore. csidlw@nus.edu.sg.
3
Department of Haematology, Singapore General Hospital, Singapore, Singapore.
4
Division of Hematology/Oncology, Cedars-Sinai Medical Center, UCLA School of Medicine, Los Angeles, USA.
5
Cancer Science Institute of Singapore, National University of Singapore, Singapore, Singapore. csiyangh@nus.edu.sg.

Abstract

BACKGROUND:

Clonal VDJ rearrangement of B/T cell receptors (B/TCRs) occurring during B/T lymphocyte development has been used as a marker to track the clonality of B/T cell populations.

METHODS:

We systematically profiled the B/T cell receptor repertoire of 936 cancer cell lines across a variety of cancer types as well as 462 Epstein-Barr Virus (EBV) transformed normal B lymphocyte lines using RNA sequencing data.

RESULTS:

Rearranged B/TCRs were readily detected in cell lines derived from lymphocytes, and subclonality or potential biclonality were found in a number of blood cancer cell lines. Clonal BCR/TCR rearrangements were detected in several blast phase CML lines and unexpectedly, one gastric cancer cell line (KE-97), reflecting a lymphoid origin of these cells. Notably, clonality was highly prevalent in EBV transformed B lymphocytes, suggesting either transformation only occurred in a few B cells or those with a growth advantage dominated the transformed population through clonal evolution.

CONCLUSIONS:

Our analysis reveals the complexity and heterogeneity of the BCR/TCR rearrangement repertoire and provides a unique insight into the clonality of lymphocyte derived cell lines.

KEYWORDS:

BCR/TCR receptor repertoire; Cancer cell lines; EBV lymphocytes

PMID:
30285677
PMCID:
PMC6167786
DOI:
10.1186/s12885-018-4840-5
[Indexed for MEDLINE]
Free PMC Article

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