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Cell Rep. 2018 Oct 2;25(1):259-269.e5. doi: 10.1016/j.celrep.2018.09.007.

Elongation/Termination Factor Exchange Mediated by PP1 Phosphatase Orchestrates Transcription Termination.

Author information

1
Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK.
2
Department of Chemistry, University of Oxford, Oxford OX1 3QU, UK.
3
Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK; Department of Chemistry, University of Oxford, Oxford OX1 3QU, UK.
4
Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK. Electronic address: lidia.vasilieva@bioch.ox.ac.uk.

Abstract

Termination of RNA polymerase II (Pol II) transcription is a key step that is important for 3' end formation of functional mRNA, mRNA release, and Pol II recycling. Even so, the underlying termination mechanism is not yet understood. Here, we demonstrate that the conserved and essential termination factor Seb1 is found on Pol II near the end of the RNA exit channel and the Rpb4/7 stalk. Furthermore, the Seb1 interaction surface with Pol II largely overlaps with that of the elongation factor Spt5. Notably, Seb1 co-transcriptional recruitment is dependent on Spt5 dephosphorylation by the conserved PP1 phosphatase Dis2, which also dephosphorylates threonine 4 within the Pol II heptad repeated C-terminal domain. We propose that Dis2 orchestrates the transition from elongation to termination phase during the transcription cycle by mediating elongation to termination factor exchange and dephosphorylation of Pol II C-terminal domain.

KEYWORDS:

C-terminal domain; CID; CTD; CTD interacting domain; CTD phosphorylation; PP1 phosphatase; RNA polymerase II; Spt5; transcription termination

PMID:
30282034
PMCID:
PMC6180485
DOI:
10.1016/j.celrep.2018.09.007
[Indexed for MEDLINE]
Free PMC Article

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