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Ren Fail. 2018 Nov;40(1):498-505. doi: 10.1080/0886022X.2018.1497517.

Effect of apigenin on apoptosis induced by renal ischemia/reperfusion injury in vivo and in vitro.

Wang X1,2,3, Wang W1,2, Wang JZ1, Yang C1, Liang CZ1,2.

Author information

1
a Department of Urology , The First Affiliated Hospital of Anhui Medical University , Hefei , PR China.
2
b Institute of Urology , Anhui Medical University , Hefei , PR China.
3
c Department of Urology, Fuyang People's Hospital , Fuyang , PR China.

Abstract

OBJECTIVES:

This study aims to investigate the effects and molecular mechanisms of apigenin (ApI) on renal ischemia/reperfusion (I/R) injury in vivo and in vitro.

METHODS:

In vivo, the left renal artery was clamped for 45 min and the right kidney was removed to study renal I/R injury on Sprague-Dawley (SD) rats. ApI was injected at 60 min before renal ischemia. In vitro, renal tubular epithelial cells (HK-2) were pretreated with or without ApI (20 uM) for 60 min and then treated with hypoxia/reoxygenation (H/R). Renal function, histology, cells apoptosis, and cell viability were tested. Furthermore, the potential molecular mechanisms were assessed.

RESULTS:

ApI pretreatment could significantly alleviated the renal function and the pathological damage as well as cells apoptosis after I/R injury. Meanwhile, ApI treatment protects H/R induced HK-2 cell apoptosis in vitro. The results of Western blot showed that ApI significantly increased the expressions of B-cell lymphoma 2 (Bcl-2) and phosphor-AKt (p-AKt), Phosphoinositide 3-kinase (PI3K), while down-regulated the expressions of Caspase3 and Bax induced by H/R injury.

CONCLUSIONS:

ApI pretreatment can protect renal function against I/R injury and prevent renal tubular cells from apoptosis in vivo and in vitro which might through PI3K/Akt mediated mitochondria-dependent apoptosis signaling pathway.

KEYWORDS:

AKt; Apigenin; Bcl-2; apoptosis; renal ischemia/reperfusion (I/R) injury

PMID:
30278824
PMCID:
PMC6171452
DOI:
10.1080/0886022X.2018.1497517
[Indexed for MEDLINE]
Free PMC Article

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