Abstract
We report site-specific triple click labeling for DNA and RNA in a one-pot setup by performing inverse electron demand Diels-Alder reaction and strain-promoted and copper catalyzed click reactions sequentially. Our methodology can be applied to long and short nucleic acids alike, and could have broader potential for labeling other (bio-)molecules.
MeSH terms
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Alkynes / chemical synthesis
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Alkynes / chemistry
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Azides / chemical synthesis
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Azides / chemistry
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Catalysis
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Click Chemistry / methods*
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Copper / chemistry
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Cycloaddition Reaction / methods*
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DNA / chemical synthesis
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DNA / chemistry*
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Fluorescent Dyes / chemical synthesis
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Fluorescent Dyes / chemistry*
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RNA / chemical synthesis
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RNA / chemistry*
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Staining and Labeling / methods*
Substances
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Alkynes
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Azides
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Fluorescent Dyes
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RNA
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Copper
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DNA