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Chemistry. 2018 Oct 22;24(59):15761-15765. doi: 10.1002/chem.201803217. Epub 2018 Oct 1.

Fluorinated Carbohydrates as Lectin Ligands: Synthesis of OH/F-Substituted N-Glycan Core Trimannoside and Epitope Mapping by 2D STD-TOCSYreF NMR spectroscopy.

Author information

1
NMR Facility, CIC bioGUNE, Bizkaia Technology Park, Bld 800, 48170, Derio, Spain.
2
Centre for Synthesis and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland.
3
Molecular Recognition and Host-Pathogen Interactions, CIC bioGUNE, Bizkaia Technology Park, Bld 800, 48170, Derio, Spain.
4
Tierärztliche Fakultät, Institut für Physiologische Chemie, Ludwig-Maximilians-Universität München, Veterinärstr. 13, 80539, München, Germany.

Abstract

Glycan-protein interactions play an important role in a broad range of physiological processes, raising interest to elucidate the structural interplay. Yet, their dynamic nature limits the analysis by crystallography, whereas NMR spectroscopy suffers from the low 1 H dispersion of glycans. Therefore, their sparse fluorination and NMR screening by 1D Saturation Transfer Difference with relay to 19 F (STDreF) was previously proposed to exploit the superior dispersion in 19 F NMR spectroscopy. A new 2D STD-TOCSYreF experiment is presented here that enables comprehensive epitope mapping of fluorinated glycans by combining the spectral resolution of 19 F with the spatial resolution and coverage of 1 H. For an illustration, the 2-deoxy-2-fluoro derivative of the N-glycan core trimannoside was synthesised and its recognition of Pisum sativum agglutinin by either of the two terminal mannose residues was confirmed. Going beyond the crystallographic information, the 2D STD-TOCSYreF spectrum moreover visualised collateral contacts from the branching mannose and allowed to assess the ratio of both co-existing binding modes through the α1,3- (67 %) and α1,6-linked (33 %) terminal mannose moieties.

KEYWORDS:

19F NMR spectroscopy; epitope mapping; fluorine; glycan; sugar code

PMID:
30276889
DOI:
10.1002/chem.201803217

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