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Int J Med Sci. 2018 Aug 10;15(12):1341-1348. doi: 10.7150/ijms.27543. eCollection 2018.

Thrombopoietin could protect cerebral tissue against ischemia-reperfusion injury by suppressing NF-κB and MMP-9 expression in rats.

Author information

1
Department of Neurology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China.
2
Department of Neurology, The First Affiliated Hospital of Henan University of Science and Technology.
3
National Clinical Research Center for Mental Disorders, the Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China.

Abstract

Objective: To determine the neuroprotective effects and underpinning mechanisms of thrombopoietin (TPO), Matrix Metalloproteinase-9(MMP-9) and Nuclear Factor-κB (NF-κB) after focal cerebral ischemia-reperfusion in rats. Methods: Male rats underwent 2 hours of right middle cerebral artery occlusion (MCAO) followed by 22 hours of reperfusion. PBS or TPO (0.1ug/kg) was administered from caudal vein before reperfusion. Neurologic deficits, brain edema, Evans blue (EB) extravasation, NF-κB and MMP-9 expression were subsequently examined. Results: Ischemia-reperfusion injury produced a large area of edema. TPO significantly reduced edema and alleviated neurologic deficits after ischemia-reperfusion. Ischemia-induced increases of NF-κB, MMP-9 and Evans blue extravasation were reduced by TPO intervention. Conclusion: TPO improved neurological function and ameliorated brain edema after stroke, partly by reducing the ischemia-induced increase of NF-κB and MMP-9.

KEYWORDS:

Ischemia-Reperfusion (IR); Matrix Metalloproteinase-9 (MMP-9); Nuclear factor-κB (NF-κB); Thrombopoietin (TPO)

Conflict of interest statement

Competing Interests: The authors have declared that no competing interest exists.

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